PREDICTING PROTEIN CRYSTALLIZATION FROM A DILUTE-SOLUTION PROPERTY
PREDICTING PROTEIN CRYSTALLIZATION FROM A DILUTE-SOLUTION PROPERTY
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DOI：
10.1107/s0907444994001216
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发表时间：
1994-07-01
期刊：
影响因子：
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通讯作者：
WILSON, WW
中科院分区：
文献类型：
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作者：
GEORGE, A;WILSON, WW
从静态光散射测量得到的稀溶液参数提出作为蛋白质结晶实验的预测。渗透第二维里系数，B22，已被测量的各种蛋白质在溶剂中，已知促进结晶和B22的值被发现位于一个相当窄的范围内，我们称之为结晶槽。已知不利于蛋白质结晶的溶液条件导致B22值远远超出结晶槽。
A dilute solution parameter obtained from static light-scattering measurements is proposed as a predictor for protein crystallization experiments. The osmotic second virial coefficients, B22, have been measured for a variety of proteins in solvents that are known to promote crystallization and the values for B22 were found to lie within a fairly narrow range which we refer to as a crystallization slot. Solution conditions which were known not to favor crystallization of the proteins resulted in B22 values well outside the crystallization slot.