Analysis of Comparison of Human Meibomian Lipid Films and Mixtures with Cholesteryl Esters In Vitro Films using High Resolution Color Microscopy.
Analysis of Comparison of Human Meibomian Lipid Films and Mixtures with Cholesteryl Esters In Vitro Films using High Resolution Color Microscopy.
复制标题
使用高分辨率彩色显微镜分析体外人睑板腺脂质膜和胆固醇酯混合物的比较。
DOI：
10.1167/iovs.12-10022
复制
发表时间：
2012
影响因子：
4.4
通讯作者：
King-Smith,PEwen
中科院分区：
文献类型：
--
作者：
Millar,ThomasJ;King-Smith,PEwen
目的：使用高分辨率彩色显微镜（HRCM）对泪液的脂质层进行了体内研究。这些实验的目的是通过将HRCM应用于体外睑板脂膜来进一步了解脂层的结构。方法：将人睑板脂、神经酸胆固醇酯、棕榈酸胆固醇酯或其混合物的膜铺展在朗缪尔槽上。当膜被压缩到不同的表面压力时，使用HRCM监测膜的变化。还研究了白蛋白对睑板脂膜的渗透。结果：少量的睑板脂质在低压下形成非常薄的膜，估计为5.2 nm厚。压缩使胶片上出现斑点。在较高浓度下，微透镜是薄膜的一个特征。胆固醇神经酸盐形成了一个多层的浮油，不随表面压力的变化。胆固醇棕榈酸酯形成在高压缩下塌陷的硬膜。胆固醇神经酸酯和睑板脂的混合物表明，它们混合以增加表面压力高于单个组件。HRCM还可观察到白蛋白穿透睑板脂膜。结论：HRCM结合体外表面压力测量使用朗缪尔槽是有用的建模睑板脂质膜。膜通常类似于体内膜的脂质层的外观。这些数据表明，脂质层可能是最好的建模作为一个双膜含有液晶阵列。
Purpose.: The lipid layer of the tears has been studied in vivo using high resolution color microscopy (HRCM). The purpose of these experiments was to gain further insight into the structure of the lipid layer by applying HRCM to in vitro meibomian lipid films.Methods.: Films of human meibomian lipids, cholesteryl nervonate, cholesteryl palmitate, or their mixtures, were spread on a Langmuir trough. Changes to the films were monitored using HRCM as the films were compressed to different surface pressures. The penetration of albumin into a meibomian lipid film also was studied.Results.: Small amounts of meibomian lipids at low pressures formed very thin films estimated to be 5.2 nm thick. Compression caused spots to appear in the films. At higher concentrations, micro lenses were a feature of the film. Cholesteryl nervonate formed a multilayered oil slick that did not change with surface pressure. Cholesteryl palmitate formed a stiff film that collapsed at high compression. Mixtures of cholesteryl nervonate and meibomian lipids showed that they mixed to increase surface pressures above that of the individual components. HRCM also allowed albumin to be seen penetrating the meibomian lipid film.Conclusions.: HRCM combined with in vitro surface pressure measurements using a Langmuir trough is useful for modeling meibomian lipid films. The films often resemble the appearance of the lipid layer of in vivo films. The data indicate that the lipid layer might be modeled best as a duplex film containing an array of liquid crystals.