Subfertility and defective folliculogenesis in female mice lacking androgen receptor
Subfertility and defective folliculogenesis in female mice lacking androgen receptor
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DOI：
10.1073/pnas.0404372101
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发表时间：
2004-08-03
影响因子：
11.1
通讯作者：
Chang, CS
中科院分区：
文献类型：
--
作者：
Hu, YC;Wang, PH;Chang, CS
雄激素受体（AR）在女性生育力和卵巢功能中的作用仍不清楚。在这里，我们报告了缺乏AIR（AR（-/-））的雌性小鼠的产生及其对生殖系统的影响。雌性AR（-/-）小鼠表现正常，但表现出较长的发情周期和生育能力下降。性成熟AR（-/-）雌性的卵巢显示黄体数量显著减少。超数排卵处理后，AR（-/-）卵巢产生的卵母细胞较少，黄体也较少。在围排卵期，AR（-/-）排卵前卵泡发生强烈的颗粒细胞凋亡事件，同时下调p21和孕酮受体的表达。此外，AR（-/-）雌性的卵丘细胞-卵母细胞复合体的缺陷构象意味着AR（-/-）卵母细胞的受精能力较低。除了黄体酮产生不足外，子宫内膜对外源性促性腺激素的反应减少，表明AR（-/-）女性表现出黄体期缺陷。总之，这些数据提供了体内证据表明，空气中起着重要的作用，在女性生殖。
The roles of the androgen receptor (AR) in female fertility and ovarian function remain largely unknown. Here we report on the generation of female mice lacking AIR (AR(-/-)) and the resulting influences on the reproductive system. Female AR(-/-) mice appear normal but show longer estrous cycles and reduced fertility. The ovaries from sexually mature AR(-/-) females exhibited a marked reduction in the number of corpora lutea. After superovulation treatment, the AR(-/-) ovaries produced fewer oocytes and also showed fewer corpora lutea. During the periovulatory period, an intensive granulosa apoptosis event occurs in the AR(-/-) preovulatory follicles, concurrent with the down-regulation of p21 and progesterone receptor expression. Furthermore, the defective conformation of the cumulus cell-oocyte complex from the AR(-/-) females implies a lower fertilization capability of the AR(-/-) oocytes. In addition to insufficient progesterone production, the diminished endometrial growth in uteri in response to exogenous gonadotropins indicates that AR(-/-) females exhibit a luteal phase defect. Taken together, these data provide in vivo evidence showing that AIR plays an important role in female reproduction.