Development of a Stable Isotope Dilution Analysis for the Quantification of the Bacillus cereus Toxin Cereulide in Foods
Development of a Stable Isotope Dilution Analysis for the Quantification of the Bacillus cereus Toxin Cereulide in Foods
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DOI：
10.1021/jf9033046
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发表时间：
2010-02-10
影响因子：
6.1
通讯作者：
Ehling-Schulz, Monika
中科院分区：
文献类型：
--
作者：
Bauer, Tobias;Stark, Timo;Ehling-Schulz, Monika
越来越多的严重食源性中毒是由一种高度稳定的缩酚肽引起的，称为催吐蜡样芽孢杆菌菌株产生的催吐素。由于催泪素对人类健康构成风险，因此必须开发适当的方法来分析这种毒素。因此，通过使用B的生物合成方法制备了催吐利参比物质及其C-13（6）-同位素体。蜡状芽孢杆菌培养，随后进行快速但有效的下游纯化。通过液相色谱-飞行时间质谱、液相色谱-串联质谱和一维/二维核磁共振谱确证结构后，建立了以13（6）-催吐素为内标物的稳定同位素稀释分析法（SIDA）。进行了验证实验，并将数据与使用结构相关的缬氨霉素代替C-13（6）-cereulide作为内标物的定量分析进行了比较。以相对标准偏差表示的真实性、重复性和再现性显示值
An increasing number of severe food borne intoxications are caused by a highly stable depsipeptide, named cereulide, which is produced by emetic Bacillus cereus strains. As cereulide poses a health risk to humans, the development of an appropriate method for the analysis of this toxin is mandatory. Therefore, the reference material of cereulide as well as its C-13(6)-isotopologue was prepared by means of a biosynthetic approach using a B. cereus culture, followed by a rapid but efficient downstream purification. After structure confirmation by means of liquid chromatography (LC)-time-of-flight mass spectrometry, LC-tandem mass spectrometry, and one-/two-dimensional NMR spectroscopy, a stable isotope dilution analysis (SIDA) was developed for the quantification of cereulide in foods using the C-13(6)-cereulide as the internal standard. Validation experiments were performed, and the data were compared to the quantitative analysis using the structurally related valinomycin instead of the C-13(6)-cereulide as an internal standard. Trueness, repeatability, and reproducibility expressed as relative standard deviation showed values