Accumulation of Co in Yeast Cells under Metabolically Active Condition-Implication for Role of Yeast in Migration of Radioactive Co-
Accumulation of Co in Yeast Cells under Metabolically Active Condition-Implication for Role of Yeast in Migration of Radioactive Co-
复制标题
代谢活跃条件下酵母细胞中钴的积累——酵母在放射性钴迁移中的作用的意义
DOI：
10.1080/18811248.2011.9711808
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
他2名
中科院分区：
文献类型：
--
作者：
N. KOZAI;T. OHNUKI;F. SAKAMOTO;Y. SUZUKI;K. TANAKA;他2名
The accumulation and change in the chemical states of Co have been studied to elucidate the role of the yeastSaccharomyces cerevisiaein the migration of radioactive cobalt in the environment. The yeast was grown in a solution containing Co(II) ions, a carbon source, and essential elements (metabolically active condition). For comparison, an adsorption experiment of Co(II) ions on the yeast cells under resting condition without essential elements was performed. Time courses of Co concentration in the solution, in the cells, and chemical states of the accumulated Co were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES), particle-induced X-ray emission (PIXE), and X-ray absorption fine structure (XAFS) analyses. The time courses of Co concentration in the solution showed that a higher amount of Co was accumulated by the yeast cells under the metabolically active condition than under the resting one. PIXE analyses showed the concurrent accumulation of Fe with Co accumulation under the metabolically active condition, suggesting the intracellular accumulation of Co. XAFS analyses showed that the k3-weighted extended-XAFS functions and the radial structural function of Co accumulated by the yeast cells under the metabolically active condition are similar to those under the resting condition, indicating that the chemical states of the accumulated Co were nearly the same between both conditions. These results indicate that the yeast performs better retardation of the migration of Co under the metabolically active condition than under the resting one.
