Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera.
Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera.
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DOI：
10.1186/1471-213x-7-73
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发表时间：
2007-06-25
影响因子：
--
通讯作者：
Zhao XF
中科院分区：
文献类型：
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作者：
Dong DJ;He HJ;Chai LQ;Jiang XJ;Wang JX;Zhao XF
幼虫蜕皮和变态是全变态昆虫生命周期中重要的生理过程。我们使用抑制消减杂交（SSH）来鉴定幼虫蜕皮和变态过程中差异表达的基因。我们在不同发育阶段的组织之间进行了 SSH，包括蜕皮和喂养 6 龄幼虫、变态幼虫和喂养 5 龄幼虫，以及喂养 5 龄和变态幼虫。鉴定出一百个表达序列标签 (EST)，其中包括 73 个与已知基因相似的推定基因和 27 个未知 EST。通过斑点印迹、Northern 印迹和 RT-PCR 进一步表征 SSH 结果。发现十一个基因的表达水平在幼虫蜕皮或变态过程中发生变化，表明在这些过程中发挥功能作用。这些结果提供了一组在幼虫蜕皮或变态期间特异性表达的新基因，这些基因是进一步研究这些阶段特异性基因在幼虫蜕皮和变态期间的调控机制的候选基因
Larval molting and metamorphosis are important physiological processes in the life cycle of the holometabolous insect. We used suppression subtractive hybridization (SSH) to identify genes differentially expressed during larval molting and metamorphosis. We performed SSH between tissues from a variety of developmental stages, including molting 5th and feeding 6th instar larvae, metamorphically committed and feeding 5th instar larvae, and feeding 5th instar and metamorphically committed larvae. One hundred expressed sequence tags (ESTs) were identified and included 73 putative genes with similarity to known genes, and 27 unknown ESTs. SSH results were further characterized by dot blot, Northern blot, and RT-PCR. The expression levels of eleven genes were found to change during larval molting or metamorphosis, suggesting a functional role during these processes. These results provide a new set of genes expressed specifically during larval molt or metamorphosis that are candidates for further studies into the regulatory mechanisms of those stage-specific genes during larval molt and metamorphosis