Double flower formation in Tricyrtis macranthopsis is related to low expression of AGAMOUS ortholog gene
Double flower formation in Tricyrtis macranthopsis is related to low expression of AGAMOUS ortholog gene
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DOI：
10.1016/j.scienta.2015.06.050
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发表时间：
2015-09
影响因子：
4.3
通讯作者：
A. Sharifi;K. Oizumi;S. Kubota;A. Bagheri;S. M. Shafaroudi;M. Nakano;A. Kanno
中科院分区：
文献类型：
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作者：
A. Sharifi;K. Oizumi;S. Kubota;A. Bagheri;S. M. Shafaroudi;M. Nakano;A. Kanno
含有大量花瓣的复杂花朵作为观赏植物尤其珍贵。重花毛菖蒲雄蕊和心皮向花瓣状器官转化的过程与agamous （AG）基因表达有关。因此我们从mt中分离出agamousgene。名为asTrimAG的大红花。由于trimagine的表达仅限于3和4轮，并且trimagine的异位表达与转基因拟南芥中其他同源基因的过表达引起相同的表型，因此该基因与其同源基因具有相似的功能。在双花t里。trimage在两个内生殖轮系（雄蕊和心皮）中的表达水平降低，导致生殖器官被花被替换，开花不确定。基因组DNA的Southern blot分析表明，重花栽培品种与野生型之间无显著差异。启动子和agintron II序列分析未发现重花品种中存在任何严重突变序列。这些结果表明，在第3和第4轮中，trimag表达减少可能是由于控制sagamous表达的调控网络中的一个基因突变或trimagpromoter /内含子序列甲基化导致转录沉默引起的。
Complex flowers containing large numbers of petals are especially prized as ornamental plants. Double-floweredTricyrtis macranthopsis, in which stamens and carpels are converted to petaloid organs, is related toAGAMOUS(AG) gene expression. So we isolatedAGAMOUSgene fromT. macranthopsis, named asTrimAG. SinceTrimAGexpression was limited to whorls 3 and 4 and ectopic expression ofTrimAGinArabidopsis thalianacaused the same phenotype as overexpression of otherAGorthologs in transgenicArabidopsis, this gene has similar function as itsAGorthologs. In the double-floweredT. macranthopsiscultivar, the level ofTrimAGexpression was reduced in the two inner reproductive whorls (stamens and carpels) and caused reproductive organ replacement with petaloid tepals and floral indeterminacy. Southern blot analysis of genomic DNA indicated that there were no significant differences between the double-flowered cultivar and wild-type plants. Analysis of promoter andAGintron II sequences did not identify any critically mutated sequences in the doubled-flowered cultivar. These results suggest that reducedTrimAGexpression in whorls 3 and 4 might be caused by mutation in one of the genes in regulatory network which controlsAGAMOUSexpression or transcriptional silencing by methylation ofTrimAGpromoter/intron sequences.