Characterization of Cardiac Size and Function in SHRSP Z-Leprfa/IzmDmcr Rats, a New Animal Model of Metabolic Syndrome
Characterization of Cardiac Size and Function in SHRSP Z-Leprfa/IzmDmcr Rats, a New Animal Model of Metabolic Syndrome
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DOI：
10.1248/bpb.33.1971
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发表时间：
2010-12-01
影响因子：
2
通讯作者：
Shinozuka, Kazumasa
中科院分区：
文献类型：
--
作者：
Tada, Yukari;Kagota, Satomi;Shinozuka, Kazumasa
在代谢综合征中观察到心脏结构和功能异常，然而，在SHRSP Z-Lepri（fa）/IzmDmcr大鼠中尚未研究这种变化代谢综合征的（SHRSP-脂肪）模型在此，我们比较了这些大鼠与其瘦的同窝出生大鼠（SHRSP）和血压正常的对照Wistar-Kyoto大鼠（WKY）的心脏大小和血流动力学功能。通过尾袖法测定心率和收缩压（SBP），通过体积描记法测定心输出量（CO）、皮下外周血流量（BF）和每搏输出量（SV），通过超声心动图测定收缩和舒张功能。清醒的SHRSP-脂肪组心率低于SHRSP组，SHRSP-脂肪组和SHRSP-脂肪组的离体心房搏动率低于WKV组，SHRSP-脂肪组的心房重量大于其他组，SHRSP-脂肪组和SHRSP-脂肪组的心室重量大于WKY组，SHRSP-脂肪组的心房重量与心率或搏动率呈显著负相关与SHRSP相似，SHRSP-脂肪组SBP、CO、BF和SV增加，SHRSP-脂肪组减速时间增加，E/A比值降低，缩短分数保持不变。SHRSP-脂肪组心房和心室I型胶原表达增加，伴有代谢综合征的SHRSP-脂肪组出现心脏改变，包括心率减慢，心室舒张功能障碍和纤维化，SHRSP-fatty大鼠心房肥大模型可能是研究代谢综合征心脏异常的一种有用的动物模型
Cardiac structural and functional abnormalities are observed in metabolic syndrome However, such changes have not been investigated in the SHRSP Z-Lepri(fa)/IzmDmcr rat (SHRSP-fatty) model of metabolic syndrome Here we compare cardiac size and hemodynamic function in these rats with their lean littermates (SHRSP) and normotensive control Wistar-Kyoto rats (WKY) In male 16-week-old SHRSP-fatty, we determined heart rate and systolic blood pressure (SBP) by tail-cuff, cardiac output (CO), subcutaneous peripheral blood flow (BF) and stroke volume (SV) by plethysmography, and systolic and diastolic functions by echocardiography We also assessed weight and collagen type I expression by Western blot in Isolated atrium and ventricle, and beat rate in isolated atrial preparation by myography Heart rate was lower in conscious SHRSP-fatty than SHRSP, and the beat rate of Isolated atria was lower in SHRSP-fatty and SHRSP than that of WKV Atrial weight was larger in SHRSP-fatty than others Ventricular weight of SHRSP-fatty and SHRSP was larger than WKY There were significant Inverse correlations between atrial weight and heart rate or beat rate in SHRSP-fatty SBP, CO, BF and SV were Increased in SHRSP-fatty similarly to SHRSP Increased deceleration time and decreased E/A ratio, and preserved fractional shortening were observed in SHRSP-fatty Expressions of collagen type I were Increased in atria and ventricle of SHRSP-fatty SHRSP-fatty with metabolic syndrome exhibit cardiac changes, including slowed heart rate, ventricular diastolic dysfunction, and fibrosis, and atrial enlargement SHRSP-fatty may be a useful rat model to study on cardiac abnormalities in metabolic syndrome