The effect of grafting method on the colloidal stability and in vitro cytotoxicity of carboxymethyl dextran coated magnetic nanoparticles
The effect of grafting method on the colloidal stability and in vitro cytotoxicity of carboxymethyl dextran coated magnetic nanoparticles
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DOI：
10.1039/c0jm01504k
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发表时间：
2010-01-01
影响因子：
--
通讯作者：
Rinaldi, Carlos
中科院分区：
文献类型：
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作者：
Creixell, Mar;Herrera, Adriana P.;Rinaldi, Carlos
在生物相关缓冲液和MCF-7细胞培养物中研究了吸附或共价结合羧甲基葡聚糖（CMDx）的氧化铁纳米颗粒的胶体稳定性和细胞毒性。还与氨基丙基硅烷涂覆的纳米颗粒进行了比较，氨基丙基硅烷涂覆的纳米颗粒是获得具有共价结合的CMDx的颗粒的中间步骤。具有共价结合的CMDx的颗粒在所有缓冲液和细胞培养物中胶体稳定。相反，具有氨丙基硅烷和吸附的CMDx的颗粒在测试条件下沉淀。氨基丙基硅烷涂覆的纳米颗粒仅在去离子水中和pH < 5中稳定。具有吸附的CMDx的纳米颗粒由于在磷酸盐的存在下CMDx的解吸而沉淀。在没有磁场的情况下，具有氨丙基硅烷和吸附的CMDx的纳米颗粒比具有共价结合的CMDx的纳米颗粒具有更大的细胞毒性，这与其较差的胶体稳定性相关。与未处理的样品相比，两种CMDx包被的纳米颗粒在通过磁流体热疗（MFH）降低MCF-7癌细胞活力方面同样有效，达到约4- 6%的水平。本研究说明了接枝方法在获得适用于生物医学应用的纳米粒子方面的重要性。
The colloidal stability and cytotoxicity of iron oxide nanoparticles coated with adsorbed or covalently-bound carboxymethyl dextran (CMDx) was studied in biologically relevant buffers and in MCF-7 cell culture. Comparisons were also made with aminopropylsilane coated nanopartciles which are an intermediate step in obtaining the particles with covalently bound CMDx. Particles with covalentlybound CMDx were colloidally stable in all the buffers and in cell culture. Conversely, particles with aminopropylsilane and adsorbed CMDx precipitated under the conditions tested. Aminopropylsilane coated nanoparticles were only stable in deionized water and pH < 5. Nanoparticles with adsorbed CMDx precipitate due to desorption of CMDx in the presence of phosphates. Nanoparticles with aminopropylsilane and adsorbed CMDx were significantly more cytotoxic in the absence of magnetic field than nanoparticles with covalently-bound CMDx, which correlates with their poor colloidal stability. Both CMDx coated nanoparticles were equally effective in decreasing MCF-7 cancer cell viability by magnetic fluid hyperthermia (MFH), to levels of around 4-6%, compared to untreated samples. This study illustrates the importance of grafting method on obtaining nanoparticles suitable for biomedical applications.