Sodium Polyanethol Sulfonate Modulates Natural Transformation of SigH-Expressing Staphylococcus aureus
Sodium Polyanethol Sulfonate Modulates Natural Transformation of SigH-Expressing Staphylococcus aureus
复制标题
聚茴香醇磺酸钠调节表达 SigH 的金黄色葡萄球菌的自然转化
DOI：
10.1007/s00284-017-1409-5
复制
发表时间：
2017
影响因子：
2.6
通讯作者：
Morikawa Kazuya
中科院分区：
文献类型：
--
作者：
Nguyen Le Thuy Thi;Takemura Aya J.;Ohniwa Ryosuke L.;Saito Shinji;Morikawa Kazuya
金黄色葡萄球菌天然遗传能力所需基因的表达受转录因子SigH控制然而，即使在SigH表达细胞中，DNA转化效率也根据培养条件而变化。我们在这里报告，在感受态诱导培养基（CS2培养基）中生长的细胞表现出扩大的形态与解体的细胞壁。值得注意的是，自溶抑制剂，聚茴香脑磺酸钠（SPS），促进转化CS2培养基中的剂量依赖性的方式，这表明在转化的细胞壁代谢的参与。然而，在TSB中生长的细胞的转化效率并没有通过对细胞壁的物理或酶促损伤而提高。
Expression of genes required for natural genetic competence inStaphylococcus aureusis controlled by an alternative transcription sigma factor, SigH. However, even in the SigH-expressing cells, the DNA transformation efficiency varies depending on culture conditions. We report here that cells grown in the competence-inducing medium (CS2 medium) exhibit enlarged morphology with disintegrated cell walls. Notably, an autolysis inhibitor, Sodium Polyanethol Sulfonate (SPS), facilitated transformation in CS2 medium in a dose-dependent manner, suggesting the involvement of the cell wall metabolism in transformation. However, the transformation efficiency of cells grown in TSB was not improved by physical or enzymatic damage on the cell walls.