Polysaccharide from Artocarpus heterophyllus Lam. (jackfruit) pulp modulates gut microbiota composition and improves short-chain fatty acids production
Polysaccharide from Artocarpus heterophyllus Lam. (jackfruit) pulp modulates gut microbiota composition and improves short-chain fatty acids production
复制标题
来自木菠萝的多糖。
DOI：
10.1016/j.foodchem.2021.130434
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发表时间：
2021
期刊：
影响因子：
8.8
通讯作者：
Gang Wu
中科院分区：
文献类型：
--
作者：
Kexue Zhu;Haofei Fan;Shunjiang Zeng;Shaoping Nie;Yanjun Zhang;Lehe Tan;Chuan Li;Fei Xu;Qibing Liu;Gang Wu
本研究旨在探讨山菖蒲多糖的作用。JFP-Ps对小鼠肠道菌群组成和短链脂肪酸产生的影响。采用PCR扩增V3和V4区16S rRNA基因的微生物群落，在Illumina MiSeq PE250平台上测序，并采用多元统计方法进行分析。采用气相色谱法（GC）和火焰电离检测器（FID）测定了短链脂肪酸（SCFAs）的浓度。结果表明，JFP-Ps显著影响了肠道细菌的水平，包括拟杆菌门、厚壁菌门、变形菌门、蓝藻门、放线菌门、微内菌门、脱铁菌门和TM7。JFP-Ps处理2周后，小鼠粪便中乙酸、丙酸、正丁酸和总短链脂肪酸浓度显著升高。这些结果表明，JFP-Ps对肠道健康有益，可以作为一种与肠道健康相关的功能成分开发。
This study aimed to investigate the effects of polysaccharide fromArtocarpus heterophyllusLam. pulp (JFP-Ps) on gut microbiota composition and short-chain fatty acids production in mice. The microbial communities of V3 and V4 region 16S rRNA gene was amplified by PCR, then sequenced on an Illumina MiSeq PE250 platform and analyzed by multivariate statistical methods. The concentrations of short-chain fatty acids (SCFAs) were measured using gas chromatography (GC) equipped with a flame ionization detector (FID). The results showed that JFP-Ps significantly affected the levels of intestinal bacteria, including Bacteroidetes, Firmicutes, Proteobacteria, Cyanobacteria, Actinobacteria, Tenericutes, Deferribacteres and TM7. The concentrations of acetic acid, propionic acid,n-butyric acid and total SCFAs in mouse feces were significantly increased by treatment with JFP-Ps for 2 weeks. These results indicate that JFP-Ps is beneficial to the gut health and can be developed as a functional ingredient in relation to gut health.