Nanoparticulate mineralized collagen scaffolds induce in vivo bone regeneration independent of progenitor cell loading or exogenous growth factor stimulation.
Nanoparticulate mineralized collagen scaffolds induce in vivo bone regeneration independent of progenitor cell loading or exogenous growth factor stimulation.
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DOI：
10.1016/j.biomaterials.2016.02.020
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发表时间：
2016-05
期刊：
影响因子：
14
通讯作者：
Lee JC
中科院分区：
文献类型：
--
作者：
Ren X;Tu V;Bischoff D;Weisgerber DW;Lewis MS;Yamaguchi DT;Miller TA;Harley BA;Lee JC
目前的骨骼再生策略通常需要共同提供支架技术、生长因子和细胞材料。然而，干细胞的分离和扩增可能耗时、昂贵，并且需要额外的收获程序。此外，引入超生理剂量的生长因子可能会导致不良的临床副作用，需要寻求刺激成骨的替代方法。在这项工作中，我们描述了一种纳米颗粒矿化胶原糖胺聚糖支架，它可以在不添加扩张的干细胞或外源生长因子的情况下诱导临界大小的兔颅骨缺损的愈合。我们证明成骨诱导的机制对应于典型 BMP 受体信号传导的增加，继发于分化过程中早期自体产生 BMP-2 和 -9 以及后期 BMP-4。因此，纳米颗粒矿化胶原糖胺聚糖支架可以为骨再生提供一种新型的无生长因子和无离体祖细胞培养的可植入方法。
Current strategies for skeletal regeneration often require co-delivery of scaffold technologies, growth factors, and cellular material. However, isolation and expansion of stem cells can be time consuming, costly, and requires an additional procedure for harvest. Further, the introduction of supraphysiologic doses of growth factors may result in untoward clinical side effects, warranting pursuit of alternative methods for stimulating osteogenesis. In this work, we describe a nanoparticulate mineralized collagen glycosaminoglycan scaffold that induces healing of critical-sized rabbit cranial defects without addition of expanded stem cells or exogenous growth factors. We demonstrate that the mechanism of osteogenic induction corresponds to an increase in canonical BMP receptor signalling secondary to autogenous production of BMP-2 and −9 early and BMP-4 later during differentiation. Thus, nanoparticulate mineralized collagen glycosaminoglycan scaffolds may provide a novel growth factor-free and ex vivo progenitor cell culture-free implantable method for bone regeneration.