Extensive genomic conservation of cattle microsatellite heterozygosity in sheep
Extensive genomic conservation of cattle microsatellite heterozygosity in sheep
复制标题
DOI：
10.1111/j.1365-2052.1997.00153.x
复制
发表时间：
1997-08-01
期刊：
影响因子：
2.4
通讯作者：
Beattie, CW
中科院分区：
文献类型：
--
作者：
deGortari, MJ;Freking, BA;Beattie, CW
我们报告了1036个牛微卫星引物对作为绵羊连锁标记的适用性评价。大约58%（605/1036）的牛引物对扩增了绵羊的基因座。67%（409/605）的扩增位点被检测为多态性。标记杂合性，等位基因数量和等位基因大小的范围显着低于绵羊比牛在这项研究中采样。然而，中值片段大小是相似的，这些数据表明，可以相对有效地构建近缘物种之间的高分辨率比较连锁图谱。
We report the evaluation of 1036 bovine microsatellite primer pairs for their suitability as linkage markers in sheep. Approximately 58% (605/1036) of bovine primer pairs amplified a locus in sheep. Sixty-seven per cent (409/605) of amplified loci were detected as polymorphic. Marker heterozygosity, allele number and range of allele sizes were significantly lower in sheep than cattle sampled in this study. However, median fragment size was similar, These data suggest that high-resolution comparative linkage maps between closely related species can be constructed relatively efficiently.