Combined detection of breast cancer biomarkers based on plasmonic sensor of gold nanorods
Combined detection of breast cancer biomarkers based on plasmonic sensor of gold nanorods
复制标题
DOI：
10.1016/j.snb.2015.08.023
复制
发表时间：
2015-12
影响因子：
8.4
通讯作者：
Shenna Chen;Qian Zhao;Lingyang Zhang;Linqian Wang;Yun-long Zeng;Haowen Huang
中科院分区：
文献类型：
--
作者：
Shenna Chen;Qian Zhao;Lingyang Zhang;Linqian Wang;Yun-long Zeng;Haowen Huang
本研究采用基于金纳米棒（GNR）的等离子体传感器，建立了检测乳腺癌血清中癌抗原15-3 （CA15-3）和铜水平的联合检测方法。通过CA15-3与GNR@CA15-3抗体之间的免疫反应诱导光信号的简单转导，即可实现肿瘤标志物CA15-3的快速检测。另一方面，铜诱导的gnr局部表面等离子体共振的变化导致了光信号的直接读出。因此，对乳腺癌人血清样品CA15-3和铜水平的联合测定进行了实际应用。结果与医院或其他标准方法给出的结果接近一致，证实了该分析具有很高的准确性。这种联合检测策略为恶性肿瘤的早期诊断和进展监测提供了潜在的应用前景。
In this study, a combined detection assay for determination of cancer antigen 15-3 (CA15-3) and copper level in breast cancer serum was developed by use of gold nanorod (GNR)-based plasmonic sensor. Rapid detection of tumor marker (CA15-3) can be accomplished through a simple transduction of optical signal induced by immune-reaction between CA15-3 and GNR@CA15-3 antibody. On the other hand, the copper-induced change of localized surface plasmon resonance of GNRs results in a direct readout of optical signal. Therefore, practical application for combined determination of CA15-3 and copper levels of human serum samples of breast cancer was performed. The results are close agreement with those given by the hospital or other standard approach, confirming this assay with high accuracy. The combined detection strategy provides potential applications for early diagnosing and monitoring the progress of malignant tumor.