Characterization of Bacterial Community Structure in a Drinking Water Distribution System during an Occurrence of Red Water
Characterization of Bacterial Community Structure in a Drinking Water Distribution System during an Occurrence of Red Water
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DOI：
10.1128/aem.00832-10
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发表时间：
2010-11-01
影响因子：
4.4
通讯作者：
Yang, Min
中科院分区：
文献类型：
--
作者：
Li, Dong;Li, Zheng;Yang, Min
细菌在偶尔出现的红水出现中的作用尚未确定。为了更好地了解驱动红水发生的机制，使用多种分子方法确定了大规模红水事件中北京水分配系统中北京水分配系统中几个功能性细菌基团的相对丰度。为三个红水样品和一个正常水样本构建了16S rRNA基因的单个克隆文库。 β，α-和γ-杆菌构成了红水和正常水样品中的主要细菌群落，与先前的报告一致。高比例的红水克隆（25.2至57.1％）与多种氧化细菌相关或密切相关，包括嗜中性粒细胞性甲壳虫菌和辅助氧基烷，酸硫硫代菌群酸硫胆氧杆菌酸氧化剂和嗜酸脂蛋白氧化脱氧化物和嗜酸脂的热氧化剂。 gallionella属占三个红水文库中所有克隆的18.7至28.6％。定量实时PCR分析表明，Gallionella spp的16S rRNA基因拷贝浓度。在（4.1 +/- 0.9）x 10（7）（平均+/-标准偏差）和（1.6 +/- 0.3）x 10（8）x 10（8）在红水中，占13.1％+/- 2.9％至17.2％至17.2％+/- 3.6％的总细菌SPP。在这些样品中。相比之下，Gallionella spp的百分比。在正常水样品中，样品为0.1％或更低（低于检测极限），表明Gallionella spp的重要作用。在红水的形成中。
The role of bacteria in the occasional emergence of red water, which has been documented worldwide, has yet to be determined. To better understand the mechanisms that drive occurrences of red water, the bacterial community composition and the relative abundance of several functional bacterial groups in a water distribution system of Beijing during a large-scale red water event were determined using several molecular methods. Individual clone libraries of the 16S rRNA gene were constructed for three red water samples and one sample of normal water. Beta-, Alpha-, and Gammaproteobacteria comprised the major bacterial communities in both red water and normal water samples, in agreement with previous reports. A high percentage of red water clones (25.2 to 57.1%) were affiliated with or closely related to a diverse array of iron-oxidizing bacteria, including the neutrophilic microaerobic genera Gallionella and Sideroxydans, the acidophilic species Acidothiobacillus ferrooxidans, and the anaerobic denitrifying Thermomonas bacteria. The genus Gallionella comprised 18.7 to 28.6% of all clones in the three red water libraries. Quantitative real-time PCR analysis showed that the 16S rRNA gene copy concentration of Gallionella spp. was between (4.1 +/- 0.9) x 10(7) (mean +/- standard deviation) and (1.6 +/- 0.3) x 10(8) per liter in red water, accounting for 13.1% +/- 2.9% to 17.2% +/- 3.6% of the total Bacteria spp. in these samples. By comparison, the percentages of Gallionella spp. in the normal water samples were 0.1% or lower (below the limit of detection), suggesting an important role of Gallionella spp. in the formation of red water.