Use of specific fluorescent antibodies for the identification of hemoglobin C in erythrocytes
Use of specific fluorescent antibodies for the identification of hemoglobin C in erythrocytes
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使用特异性荧光抗体鉴定红细胞中的血红蛋白 C
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
T. McGuire
中科院分区：
文献类型：
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作者：
T. Papayannopoulou;V. Ahern;G. Stamatoyannopoulos;G. Lim;P. Nute;T. McGuire
通过免疫马产生抗血红蛋白 C (α2β2 6Glu→Lys) 的抗体，并通过亲和层析纯化。正如根据抗体和抗原的二价所预期的，纯化的抗体在与相应抗原反应时未能产生免疫沉淀物。通过异硫氰酸荧光素偶联抗体与固定外周血涂片中的血红蛋白抗原反应，可以鉴定单个红细胞中的血红蛋白 C。具有血红蛋白 C 基因的人的红细胞在与抗 Hb C-FITC 反应后被强烈标记；没有标记含有正常血红蛋白或 Hb S 的红细胞，这表明抗 Hb C 抗体仅识别 β 链的氨基末端片段，其中 β6 位含有赖氨酸。
Antibodies against hemoglobin C (α2β2 6Glu→Lys) were produced by immunizing horses and were purified by affinity chromatography. As expected from the bivalency of both the antibody and the antigen, the purified antibodies failed to produce immunoprecipitates upon reaction with the corresponding antigens. Identification of hemoglobin C in individual erythrocytes was achieved by reacting the fluorescein isothiocyanate‐conjugated antibodies with the hemoglobin antigen in fixed smears of peripheral blood. Red cells from persons having a hemoglobin C gene were labeled strongly upon reaction with anti‐Hb C‐FITC; there was no labeling of red cells containing normal hemoglobins or Hb S, suggesting that the anti‐Hb C antibodies recognize only the amino‐terminal segment of the β chains that contain lysine in position β6.