Effects of different components of the extracellular matrix on endothelialization
Effects of different components of the extracellular matrix on endothelialization
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DOI：
10.3233/ch-168051
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发表时间：
2016-01-01
影响因子：
2.1
通讯作者：
Franke, R. P.
中科院分区：
文献类型：
--
作者：
Krueger-Genge, A.;Fuhrmann, R.;Franke, R. P.
已知心血管假体的内皮化可改善其血液相容性。因此，身体异物通常不会自发内皮化。如何改善生物材料的内皮化正在进行大量的体外研究。在这项研究中，不同成分的组织典型的细胞外基质（ECM），如层粘连蛋白，研究了纤维连接蛋白或明胶对内皮细胞单层形成和贴壁细胞抗剪切力的影响。在天然ECM上生长的细胞和在ECM的单一化组分上生长的细胞之间的单层显著不同（p < 0.001）。只有在层粘连蛋白上生长的HUVEC显示出与在ECM上生长的HUVEC相似的密度和应力纤维模式。生长在明胶或纤连蛋白包被的盖玻片上的HUVEC不太牢固地附着在基底上;经常是单个HUVEC甚至是细胞群detached. Conclusion似乎层粘连蛋白的植入物涂层支持抗剪切内皮细胞（EC）单层的形成-上级于其他ECM成分。
The endothelialization of cardiovascular prostheses is known to improve their haemocompatibility. As such bodyforeign materials often do not endothelialize spontaneously. A lot of in vitro studies are ongoing how endothelialization of biomaterials can be improved. In this study the influence of different components of a tissue-typical extracellular matrix (ECM) like laminin, fibronectin or gelatin on the formation of an endothelial cell monolayer and on the shear resistance of adherent cells on these substrates was studied.The study revealed that the density of human venous endothelial cells (HUVEC) monolayers differed markedly between cells grown on a natural ECM and cells grown on singularized components of an ECM (p < 0.001). Only HUVEC grown on laminin showed similar densities and a stress fiber pattern comparable to HUVEC grown on the ECM. HUVEC grown on gelatin- or fibronectin-coated coverslips were less firmly attached to the substrate; frequently individual HUVEC and even groups of cells detached.Concluding it seems that coating of implants with laminin supports the formation of shear resistant endothelial cell (EC) monolayer -superior to other ECM components.