Global comparison of gene expression profiles between intramuscular and subcutaneous adipocytes of neonatal landrace pig using microarray
Global comparison of gene expression profiles between intramuscular and subcutaneous adipocytes of neonatal landrace pig using microarray
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使用微阵列全面比较新生地方猪肌内和皮下脂肪细胞的基因表达谱
DOI：
10.1016/j.meatsci.2010.05.031
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发表时间：
2010-10-01
期刊：
影响因子：
7.1
通讯作者：
Jiang, Qingyan
中科院分区：
文献类型：
--
作者：
Zhou, Guixuan;Wang, Songbo;Jiang, Qingyan
本研究的目的是比较体外分离的前脂肪细胞来源的肌内脂肪细胞和皮下脂肪细胞基因表达谱的差异。胞浆甘油三酯测定表明，在分化后期，皮下脂肪细胞比肌肉内脂肪细胞积累了更多的脂肪。基因芯片分析表明，共筛选出133个差异表达基因的172个探针，其中46个基因在肌肉内脂肪细胞中高表达，87个基因在皮下脂肪细胞中高表达。实时定量聚合酶链式反应证实与脂代谢相关的基因LPL、FABP4、FABP5和OSBPL10主要在皮下脂肪细胞中表达，而BMP4和BMP7在肌肉内脂肪细胞中高表达。结果表明，皮下脂肪细胞内脂块的聚集可能与脂代谢相关基因的高表达有关，而肌内脂肪细胞中BMP4和BMP7的高水平表达提示BMPs可能参与了肌内脂肪细胞的分化。(C)2010年美国肉类科学协会。爱思唯尔有限公司出版。保留所有权利。
The objective of this study was to compare the differences of gene expression profiles between intramuscular and subcutaneous adipocytes originated from the isolated preadipocytes in vitro. Cytosolic triglyceride determination indicated that subcutaneous adipocytes accumulated more lipid than intramuscular adipocytes did at the late stage of differentiation. Microarray assay revealed that 172 probes representing 133 genes were differentially expressed, among which 46 genes were highly expressed in intramuscular adipocytes and the other 87 genes were highly expressed in subcutaneous adipocytes. Real-time PCR confirmed that genes related to lipid metabolism, such as LPL, FABP4, FABP5 and OSBPL10, were predominantly expressed in subcutaneous adipocytes, whereas BMP4 and BMP7 were highly expressed in intramuscular adipocytes. The results indicated that the accumulation of lipid mass in subcutaneous adipocytes might be due to the highly expressed genes related to lipid metabolism, and the high levels of BMP4 and BMP7 in intramuscular adipocytes suggested that BMPs might be involved in the differentiation of intramuscular adipocytes. (C) 2010 The American Meat Science Association. Published by Elsevier Ltd. All rights reserved.