Quantitative Analysis of Sympathetic and Nociceptive Innervation Across Bone Marrow Regions in Mice
Quantitative Analysis of Sympathetic and Nociceptive Innervation Across Bone Marrow Regions in Mice
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DOI：
10.1016/j.exphem.2022.07.297
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发表时间：
2022-08-01
影响因子：
2.6
通讯作者：
Takubo, Keiyo
中科院分区：
文献类型：
--
作者：
Fujita, Shinya;Morikawa, Takayuki;Takubo, Keiyo
骨髓（BM）神经支配通过直接作用于造血干细胞或改变间充质细胞和内皮细胞的生态位功能来调节造血干细胞和祖细胞（HSPCs）的动员和应激造血。然而，基底神经支配在骨区的空间分布尚未完全阐明。因此，我们的目的是表征交感神经和伤害神经分布在每个骨和BM区域使用三维定量显微镜。我们发现交感神经和伤害神经是整个脑脊膜的主要纤维。与股骨其他区域相比，股骨BM的中心部位受交感神经和伤害神经的支配更为密集。胸骨的每个区域同样受交感神经和伤害神经支配。此外，脑脊膜中大多数交感神经和伤害神经与动脉和小动脉平行，而其程度因骨类型或脑脊膜区域而异。总之，本研究以定量的方式提供了BM神经支配的空间、拓扑和功能信息，并说明交感神经和伤害神经是BM神经支配的两个主要组成部分，主要与动脉和小动脉有关。？? 2022年吧？？血液学和干细胞学会。Elsevier Inc.出版。版权所有。
Bone marrow (BM) innervation regulates the mobilization of hematopoietic stem and progenitor cells (HSPCs) from BM and stress hematopoiesis either by acting directly on HSPCs or by altering the niche function of mesenchymal and endothelial cells. However, the spatial distribution of BM innervation across bone regions is yet to be fully elucidated. Thus, we aimed to characterize the distribution of sympathetic and nociceptive nerves in each bone and BM region using three-dimensional quantitative microscopy. We discovered that sympathetic and nociceptive nerves were the major fibers throughout the BM. Compared with other femoral regions, central parts of the femoral BM were more densely innervated by both sympathetic and nociceptive nerves. Each region of the sternum was similarly innervated by sympathetic and nociceptive nerves. Further, the majority of sympathetic and nociceptive nerves in the BM ran parallel with arteries and arterioles, whereas the degree varied according to the bone type or BM region. In conclusion, this study provides spatial, topological, and functional information on BM innervation in a quantitative manner and illustrates that sympathetic and nociceptive nerves are two major components in BM innervation, mostly associated with arteries and arterioles. ?? 2022 ISEH ??? Society for Hematology and Stem Cells. Published by Elsevier Inc. All rights reserved.