Evaluation of a novel approach for peptide sequencing: laser-induced acoustic desorption combined with P(OCH(3))(2)(+) chemical ionization and collision-activated dissociation in a Fourier transform ion cyclotron resonance mass spectrometer.
Evaluation of a novel approach for peptide sequencing: laser-induced acoustic desorption combined with P(OCH(3))(2)(+) chemical ionization and collision-activated dissociation in a Fourier transform ion cyclotron resonance mass spectrometer.
复制标题
评估一种新的肽测序方法：在傅里叶变换离子回旋共振质谱仪中激光诱导声解吸结合 P(OCH(3))(2)( ) 化学电离和碰撞激活解离。
DOI：
10.1016/j.jasms.2006.10.009
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发表时间：
2007
影响因子：
3.2
通讯作者：
Kenttämaa,HilkkaI
中科院分区：
文献类型：
--
作者：
Somuramasami,Jayalakshmi;Kenttämaa,HilkkaI
一种新的质谱方法已被开发用于获得小肽的序列信息。肽作为完整的中性分子解吸到傅立叶变换离子回旋共振质谱仪（FT-ICR）通过激光诱导声解吸（LIAD）。中性肽与二甲氧基磷鎓离子P（OCH 3）2+的反应主要是通过肽与P（OCH 3）2+的加成，然后失去两个甲醇分子，从而产生组成为（peptide+P− 2 H）+的产物离子。在持续的非共振辐射碰撞激活解离（SORI-CAD）时，（肽+P− 2 H）+离子经历CO和NH= CN或H2O、CO和NH= CN的连续损失，除了常规的an-和bn-型离子外，还产生序列相关的碎片离子。在相同的条件下，类似的质子化肽的SORI-CAD主要产生常规的an-和bn-型离子。通过使用模型肽和分子轨道计算，研究了肽与P（OCH 3）2+的反应机制和（肽+P− 2 H）+离子的解离。
A novel mass spectrometric method has been developed for obtaining sequence information on small peptides. The peptides are desorbed as intact neutral molecules into a Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR) by means of laser-induced acoustic desorption (LIAD). Reactions of the neutral peptides with the dimethoxyphosphenium ion, P(OCH3)2+, occur predominantly by addition of the peptide to P(OCH3)2+followed by the loss of two methanol molecules, thus yielding product ions with the composition (peptide+P−2H)+. Upon sustained off-resonance irradiation for collision-activated dissociation (SORI-CAD), the (peptide+P−2H)+ions undergo successive losses of CO and NH=CHR or H2O, CO, and NH=CHR to yield sequence-related fragment ions in addition to the regular an- and bn-type ions. Under the same conditions, SORI-CAD of the analogous protonated peptides predominantly yields the regular an- and bn-type ions. The mechanisms of the reactions of peptides with P(OCH3)2+and the dissociation of the (peptide+P−2H)+ions were examined by using model peptides and molecular orbital calculations.