Identification of heterogeneous cell populations in normal human intervertebral disc.
Identification of heterogeneous cell populations in normal human intervertebral disc.
复制标题
DOI：
--
复制
发表时间：
1995-02
影响因子：
2.4
通讯作者：
'. MARYK.CHELBERG;'. GARRYM.BANKS;'. DOUGLASF.GEIGER;Theodore R. Oegema
中科院分区：
文献类型：
--
作者：
'. MARYK.CHELBERG;'. GARRYM.BANKS;'. DOUGLASF.GEIGER;Theodore R. Oegema
有明确的证据表明，与椎间盘相关的组织，如关节软骨，含有几种不同表型的软骨细胞群。椎间盘的组织学数据表明，纤维环和髓核可能也是如此，但这并没有直接显示出来。首次将成人非变性髓核和纤维环细胞经酶消化后回收，并在藻酸盐珠培养系统中培养6wk。细胞仍然活着，并产生基质，但不分裂。同时对培养的细胞进行硫酸软骨素、硫酸角蛋白或I型和II型胶原的染色。纤维环和髓核的大多数细胞同时产生硫酸角蛋白和硫酸软骨素(>；60%)，少数细胞只产生一种或另一种可检测到的水平，但有相当一部分细胞既不产生角蛋白又产生硫酸软骨素。这是一种具有非软骨细胞表型的细胞群的迹象。在非退变的椎间盘中，大多数纤维环细胞同时产生I型和II型胶原，但大多数髓核细胞只产生II型胶原。这些观察结果与成人椎间盘中至少有两个表型稳定的细胞群的存在是一致的，并与环状细胞的主要群体的表型不同于细胞核的观点是一致的。
There is clear evidence that tissues related to the intervertebral disc, such as articular cartilage, contain several phenotypically different chondrocytic cell populations. Histological data for the disc suggest the same may be true for the annulus fibrosus and nucleus pulposus, but this has not been shown directly. For the first time, cells from adult human nondegenerative nucleus pulposus and annulus fibrosus were recovered after enzymatic digestion and maintained in an alginate bead culture system for up to 6 wk. The cells remained viable and produced matrix, but did not divide. Cultured cells were stained simultaneously for the presence of chondroitin sulphate and keratan sulphate, or types I and II collagen. The majority of the cells from both the annulus fibrosus and the nucleus pulposus produced both keratan sulphate and chondroitin sulphate (> 60%), a few only detectable levels of one or the other, but a significant population produced neither. This is an indication of a population of cells with a nonchondrocytic phenotype. In nondegenerative discs, the majority of the annulus fibrosus cells produced both types I and II collagen but the majority of nucleus pulposus cells produced only type II collagen. These observations are consistent with the presence of at least 2 phenotypically stable populations of cells in the adult human intervertebral disc and with the view that the phenotype of the major population of the annulus is different from that of the nucleus.