Insulin-like growth factor-1 inhibition of apoptosis is associated with increased expression of the bcl-x(L) gene product.
Insulin-like growth factor-1 inhibition of apoptosis is associated with increased expression of the bcl-x(L) gene product.
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DOI：
10.1210/en.138.3.1355
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发表时间：
1997-03-01
期刊：
影响因子：
4.8
通讯作者：
LeRoith, D
中科院分区：
文献类型：
--
作者：
Parrizas, M;LeRoith, D
分化的PC12细胞依赖于生长因子的存在并在去血清培养数小时后死亡，用来检测胰岛素样生长因子-1对内源性死亡抑制蛋白Bc l-x(L)水平的调节能力。在生理浓度下，胰岛素样生长因子-1能够阻止去血清诱导的分化的PC12细胞的凋亡，以10(-8)M为最佳。我们的结果表明，胰岛素样生长因子-1对PC12细胞的保护作用与上调Bclx(L)基因和蛋白水平有关。
Differentiated PC12 cells, which become dependent on the presence of growth factors in the media and die by apoptosis after several hours of serum deprivation, were used to test the ability of IGF-1 to regulate the endogenous levels of the death-suppressing protein Bcl-x(L) IGF-1 was capable of preventing apoptosis of serum-deprived differentiated PC12 cells at physiological concentrations, with optimal results seen at 10(-8) M. Incubation with the hormone resulted in a significant increase of Bcl-x mRNA after 3-6 h incubation and a doubling of Bcl-x(L) protein levels by 24 h incubation. Our results show that the protective effect of IGF-1 in PC12 cells is associated with an up-regulation of Bcl-x(L) mRNA and protein levels.