Leukocyte cell-derived chemotaxin 2 inhibits development of atherosclerosis in mice
Leukocyte cell-derived chemotaxin 2 inhibits development of atherosclerosis in mice
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白细胞来源的趋化因子2抑制小鼠动脉粥样硬化的发展
DOI：
10.24272/j.issn.2095-8137.2019.030
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发表时间：
2019-07
影响因子：
4.9
通讯作者：
Wang J A
中科院分区：
文献类型：
--
作者：
He W M;Dai T;Chen J;Wang J A
白细胞源趋化因子2（LECT 2）是一种多功能的肝细胞因子，参与多种病理状态。然而，其在动脉粥样硬化中的作用仍不明确。在这项研究中，我们给雄性Apoe-/-小鼠喂食西方饮食15周。用油红O和苏木精染色观察和定量动脉粥样硬化病变。实时定量聚合酶链反应检测MCP-1、MMP-1、IL-8、IL-1β和TNF-α的mRNA表达水平。采用酶联免疫吸附法测定血清TNF-α、IL-1β、IL-8、MCP-1和MMP-1浓度。免疫组化法检测巨噬细胞、内皮细胞和平滑肌细胞的标志物CD 68、CD 31和α-SMA。结果显示，LECT 2降低血清中总胆固醇和低密度脂蛋白浓度，抑制动脉粥样硬化病变的发展，伴随着炎性细胞因子的减少以及MCP-1、MMP-1、TNF-α、IL-8和IL-1β mRNA丰度的降低。此外，LECT 2降低了CD 68，但增加了动脉粥样硬化病变中的α-SMA，表明平滑肌细胞增加，巨噬细胞减少。总之，LECT 2抑制了小鼠动脉粥样硬化的发展，同时降低了血清总胆固醇浓度和炎症反应。
Leukocyte cell-derived chemotaxin 2 (LECT2), a multifunctional hepatokine, is involved in many pathological conditions. However, its role in atherosclerosis remains undefined. In this study, we administered vehicle or LECT2 to male Apoe-/- mice fed a Western diet for 15 weeks. Atherosclerotic lesions were visualized and quantified with Oil-red O and hematoxylin staining. The mRNA expression levels of MCP-1, MMP-1, IL-8, IL-1β, and TNF-α were analyzed by quantitative real-time polymerase chain reaction. Serum TNF-α, IL-1β, IL-8, MCP-1, and MMP-1 concentrations were measured by enzyme-linked immunosorbent assay. CD68, CD31, and α-SMA, markers of macrophages, endothelial cells, and smooth muscle cells, respectively, were detected by immunostaining. Results showed that LECT2 reduced total cholesterol and low-density lipoprotein concentrations in serum and inhibited the development of atherosclerotic lesions, accompanied by reductions in inflammatory cytokines and lower MCP-1, MMP-1, TNF-α, IL-8, and IL-1β mRNA abundance. Furthermore, LECT2 decreased CD68, but increased α-SMA in atherosclerotic lesions, suggesting an increase in smooth muscle cells and reduction in macrophages. In summary, LECT2 inhibited the development of atherosclerosis in mice, accompanied by reduced serum total cholesterol concentration and lower inflammatory responses.
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