Expression and localization of ferritin mRNA in ameloblasts of rat incisor
Expression and localization of ferritin mRNA in ameloblasts of rat incisor
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DOI：
10.1016/s0003-9969(98)00014-4
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发表时间：
1998-05-01
影响因子：
3
通讯作者：
Kato, Y
中科院分区：
文献类型：
--
作者：
Miyazaki, Y;Sakai, H;Kato, Y
在成熟阶段，大鼠切牙的成釉细胞结合通过血流供应的铁，并将其存款到釉质的表层。在这个独特的铁转运系统中，铁蛋白在细胞中充当瞬时铁库。在这里，铁蛋白mRNA的表达和其定位在大鼠成釉器官进行了检查。在各种组织中，成釉器官表现出最高的表达铁蛋白和L-链mRNA，定量的逆转录-聚合酶链反应。用地高辛标记的cRNA探针进行原位杂交，结果表明铁蛋白H链和L链mRNA在分泌期和分泌期成釉细胞中均有大量表达，阳性杂交信号强度随切缘方向逐渐减弱。与mRNA定位不同的是，铁蛋白在分泌前或分泌期成釉细胞中免疫学检测不到，但在成熟期成釉细胞中发现，已知铁从血流中掺入其中。因此，在大鼠切牙成釉细胞的发育阶段，铁蛋白mRNA的表达先于蛋白的表达，并且铁蛋白的翻译及其半衰期可能受铁进入的控制，正如已经报道的其它细胞类型。(C)1998爱思唯尔科技有限公司版权所有。
At the maturation stage, the ameloblasts of the rat incisor incorporate iron, supplied through the bloodstream, and deposit it into the surface layer of the enamel. In this unique iron transport system, ferritin functions as a transient iron reservoir in the cells. Here the expression of ferritin mRNA and its localization in the rat enamel organ was examined. Among various tissues, the enamel organ showed the highest expression for both ferritin Hand L-chain mRNA, as quantified by reverse transcription-polymerase chain reaction. In situ hybridization using digoxigenin-labelled cRNA probes for each chain demonstrated that both ferritin H- and L-chain mRNA were abundantly expressed in presecretory and secretory ameloblasts, The intensity of the positive hybridization signal gradually decreased toward the incisal direction. Differing from the mRNA localization, ferritin protein was immunologically undetectable in presecretory or secretory ameloblasts but was found in ameloblasts at the maturation stage, into which iron is known to be incorporated from the bloodstream.Thus, the expression of ferritin mRNA precedes the protein expression in the developmental stages of rat incisor ameloblasts, and the translation of ferritin and its half-life are probably controlled by the iron entry, as has been reported for other cell types. (C) 1998 Elsevier Science Ltd. All rights reserved.