Characterization of triacylglycerol and diacylglycerol composition of plant oils using high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry
Characterization of triacylglycerol and diacylglycerol composition of plant oils using high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry
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DOI：
10.1016/s0021-9673(03)01030-6
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发表时间：
2003-08-29
影响因子：
4.1
通讯作者：
Hrubá, L
中科院分区：
文献类型：
--
作者：
Holcapek, M;Jandera, P;Hrubá, L
16种植物油（榛子、开心果、罂粟籽、杏仁、棕榈、巴西坚果、油菜籽、澳洲坚果、大豆、向日葵、亚麻籽、香青兰、月见报春花、玉米、阿马兰斯）中的三酰甘油（TG）和二酰甘油（DG）。采用大气压化学电离（APCI）和205 nm紫外检测器在2个串联的Nova-Pak C-18色谱柱上进行HPLC-MS分析。采用单一色谱柱和非水乙醇-乙腈梯度系统作为分析时间和分辨率之间的折衷，用于表征五种植物油的TG组成。应用APCI质谱鉴定所有TG和其他酰基甘油。同量异位位置异构体可以基于碎片离子的不同相对丰度来区分，所述碎片离子由脂肪酸从sn-1（3）位置与从sn-2位置相比的优选损失形成。出色的色谱分离度和宽保留窗口以及APCI质谱能够阳性鉴定含有奇数碳原子脂肪酸的TG，如十七烷酸（C17：0）和十七烷酸（C17：1）。根据离子阱质谱测量结果，提出了TG在APCI和电喷雾电离质谱中的一般裂解模式。基于在205 nm处用UV检测测量的相对峰面积来估计所分析的植物油中特定TG的相对浓度。(C)2003 Elsevier B. V.保留所有权利。
Triacylglycerols (TGs) and diacylglycerols (DGs) in 16 plant oil samples (hazelnut, pistachio, poppy-seed, almond, palm, Brazil-nut, rapeseed, macadamia, soyabean, sunflower, linseed, Dracocephalum moldavica, evening primrose, corn, amaranth. Silybum arianum) were analyzed by HPLC-MS with atmospheric pressure chemical ionization (APCI) and UV detection at 205 nm on two Nova-Pak C-18 chromatographic columns connected in series. A single chromatographic column and non-aqueous ethanol-acetonitrile gradient system was used as a compromise between the analysis time and the resolution for the characterization of TG composition of five plant oils. APCI mass spectra were applied for the identification of all TGs and other acylglycerols. The isobaric positional isomers can be distinguished on the basis of different relative abundances of the fragment ions formed by preferred losses of the fatty acid from sn-1(3) positions compared to the sn-2 position. Excellent chromatographic resolution and broad retention window together with APCI mass spectra enabled positive identification of TGs containing fatty acids with odd numbers of carbon atoms such as margaric (C17:0) and heptadecanoic (C17:1) acids. The general fragmentation patterns of TGs in both APCI and electrospray ionization mass spectra were proposed on the basis of MS" spectra measured with an ion trap analyzer. The relative concentrations of particular TGs in the analyzed plant oils were estimated on the basis of relative peak areas measured with UV detection at 205 nm. (C) 2003 Elsevier B.V. All rights reserved.