Differential Gene Expression for Age Estimation of Forensically Important Sarcophaga peregrina (Diptera: Sarcophagidae) Intrapuparial
Differential Gene Expression for Age Estimation of Forensically Important Sarcophaga peregrina (Diptera: Sarcophagidae) Intrapuparial
复制标题
法医学上重要的石食虫（双翅目：石食虫科）蛹内年龄估计的差异基因表达
DOI：
10.1093/jme/tjz137
复制
发表时间：
2020-01-01
影响因子：
2.1
通讯作者：
Guo, Yadong
中科院分区：
文献类型：
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作者：
Shang, Yanjie;Ren, Lipin;Guo, Yadong
摘要在法医昆虫学中，对最小死后间隔（PMImin）进行估算是一种重要的蝇类。准确测定尸噬石棺的发育年龄是利用尸噬石棺估计pmmin的关键任务。在幼虫发育过程中，通过形态变化和长、重、宽的变化直接确定年龄；然而，由于不可见的解剖和形态变化，对石棺顶骨内的年龄估计更为困难。石棺蜕变过程中差异表达基因（DEGs）的分析是估计石棺年龄的一种潜在方法。本研究采用实时定量聚合酶链反应（RT-qPCR）分析不同恒温（35°C、25°C和15°C）条件下游槐胞内基因表达水平的差异。此外，在不同温度下和胞内选择合适的内参基因，获得可靠有效的基因表达谱。结果表明，2个候选基因（18S rRNA和28S rRNA）是最可靠的内参基因，而4个deg （Hsp90、a - α、AFP、AFBP）有可能用于更准确地估计白腹金鸡的年龄。
Abstract Sarcophaga peregrina is an important flesh fly species for estimating the minimum postmortem interval (PMImin) in forensic entomology. The accurate determination of the developmental age is a crucial task for using necrophagous sarcophagids to estimate PMImin. During larval development, the age determination is straight forward by the morphological changes and variation of length, weight, and width; however, the age estimation of sarcophagid intrapuparial is more difficult due to anatomical and morphological changes not being visible. The analysis of differentially expressed genes (DEGs) during sarcophagid metamorphosis is a potential method for age estimation of intrapuparial. In the present study, real-time quantitative polymerase chain reaction (RT-qPCR) was used to analyze the differential gene expression level of S. peregrina intrapuparial in different constant temperatures (35°C, 25°C, and 15°C). In addition, the appropriate reference genes of S. peregrina were selected in the intrapuparial and at different temperatures to obtain reliable and valid gene expression profiles. The results indicated that two candidate genes (18S rRNA and 28S rRNA) were the most reliable reference genes, and four DEGs (Hsp90, A-alpha, AFP, AFBP) have the potential to be used to more accuracy estimate the age of S. peregrina intrapuparial.