Aspergillus lentulus sp nov., a new sibling species of A-fumigatus
Aspergillus lentulus sp nov., a new sibling species of A-fumigatus
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DOI：
10.1128/ec.4.3.625-632.2005
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发表时间：
2005-03-01
期刊：
影响因子：
--
通讯作者：
Marr, KA
中科院分区：
文献类型：
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作者：
Balajee, SA;Gribskov, JL;Marr, KA
在先前的研究中，我们鉴定了7株曲霉属的临床分离株，它们在多种培养基中孢子形成缓慢，并表现出对多种抗真菌药的体外敏感性降低，这些抗真菌药包括阿替霉素B、伊曲康唑、伏立康唑和卡泊芬净。由于线粒体细胞色素B序列和独特的随机扩增多态性DNA PCR模式的差异，这些分离株最初被认为是烟曲霉的变异株（S。A. Balajee，M.韦弗，A. Imhof，J. Gribskov，and K. A.马尔，抗菌剂。探员Chemother 48：1197-1203，2004）。本研究通过对5个基因（P-微管蛋白基因、小杆A基因、盐响应基因、线粒体细胞色素B基因和内转录间隔区）的多位点序列分型进行系统发育分析，阐明这些生物的分类地位。结果显示，七个变异分离株中的四个聚集在与A.与烟曲霉属的其他成员不同。这个新的分支，由四个成员组成，是单系与强大的自助支持蛋白质编码区进行了分析，表明一个新的物种状态下的系统发育物种的概念。表型研究表明，与烟曲霉相比，变异分离株具有较小的分生孢子头和小泡，并且不能在48 ℃下存活。我们的研究结果表明，存在一种以前未被认识的，潜在的耐药曲霉属物种，我们命名为A。小扁豆
In a prior study, we identified seven clinical isolates of an Aspergillus sp. that were slow to sporulate in multiple media and demonstrated decreased in vitro susceptibilities to multiple antifungals, including amphotericin B, itraconazole, voriconazole, and caspofungin. These isolates were initially considered to be variants of Aspergillus fumigatus because of differences in mitochondrial cytochrome b sequences and unique randomly amplified polymorphic DNA PCR patterns (S. A. Balajee, M. Weaver, A. Imhof, J. Gribskov, and K. A. Marr, Antimicrob. Agents Chemother. 48: 1197-1203, 2004). The present study was performed to clarify the taxonomic status of these organisms by phylogenetic analyses based on multilocus sequence typing of five genes (the P-tubulin gene, the rodlet A gene, the salt-responsive gene, the mitochondrial cytochrome b gene, and the internal transcribed spacer regions). Results revealed that four of the seven variant isolates clustered together in a clade very distant from A. fumigatus and distinct from other members of the A.fumigatus group. This new clade, consisting of four members, was monophyletic with strong bootstrap support when the protein-encoding regions were analyzed, indicating a new species status under the phylogenetic species concept. Phenotype studies revealed that the variant isolate has smaller conidial heads with diminutive vesicles compared to A.fumigatus and is not able to survive at 48 degrees C. Our findings suggest the presence of a previously unrecognized, potentially drug-resistant Aspergillus species that we designate A. lentulus.