The Phlebotomus papatasi systemic transcriptional response to trypanosomatid-contaminated blood does not differ from the non-infected blood meal.
The Phlebotomus papatasi systemic transcriptional response to trypanosomatid-contaminated blood does not differ from the non-infected blood meal.
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DOI：
10.1186/s13071-020-04498-0
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发表时间：
2021-01-06
影响因子：
3.2
通讯作者：
Ligoxygakis P
中科院分区：
文献类型：
--
作者：
Sloan MA;Sadlova J;Lestinova T;Sanders MJ;Cotton JA;Volf P;Ligoxygakis P
利什曼病由利什曼原虫属寄生虫引起，是一种影响全球多达800万人的疾病。寄生虫通过被感染的白蛉叮咬传播给人类和动物宿主。新的疾病控制策略需要更好地了解传播的关键步骤，即在苍蝇体内建立感染。这项工作的目的是确定与利什曼原虫感染相关的白蛉系统性转录组特征。我们使用下一代测序技术来描述当喂食单独的血液（对照）或含有以下三种锥虫之一的血液时，整个帕帕塔白蛉的转录组：donovani和Herpetomonas muscarum，后者是一种不传播给人类的寄生虫。在所研究的锥虫中，只有L. major能够成功地在宿主帕帕塔斯中建立感染。然而，在每次寄生虫污染的血粉后观察到的转录特征并不特异于特定感染的成功或失败，并且它们彼此没有差异。在未污染的血餐后，转录签名也无法区分。结果表明，白蛉认为利什曼原虫只是其微生物组景观的一个特征，任何解决传播问题的策略都应该集中在对血餐的反应上，而不是寄生虫的建立。另外，利什曼原虫可以抑制宿主的反应。这些结果将围绕通过控制昆虫体内的寄生虫来阻止传播的概念产生新的思考。
Leishmaniasis, caused by parasites of the genus Leishmania, is a disease that affects up to 8 million people worldwide. Parasites are transmitted to human and animal hosts through the bite of an infected sand fly. Novel strategies for disease control require a better understanding of the key step for transmission, namely the establishment of infection inside the fly. The aim of this work was to identify sand fly systemic transcriptomic signatures associated with Leishmania infection. We used next generation sequencing to describe the transcriptome of whole Phlebotomus papatasi sand flies when fed with blood alone (control) or with blood containing one of three trypanosomatids: Leishmania major, L. donovani and Herpetomonas muscarum, the latter being a parasite not transmitted to humans. Of the trypanosomatids studied, only L. major was able to successfully establish an infection in the host P. papatasi. However, the transcriptional signatures observed after each parasite-contaminated blood meal were not specific to success or failure of a specific infection and they did not differ from each other. The transcriptional signatures were also indistinguishable after a non-contaminated blood meal. The results imply that sand flies perceive Leishmania as just one feature of their microbiome landscape and that any strategy to tackle transmission should focus on the response towards the blood meal rather than parasite establishment. Alternatively, Leishmania could suppress host responses. These results will generate new thinking around the concept of stopping transmission by controlling the parasite inside the insect.