Higher chondrogenic potential of fibrous synovium- and adipose synovium-derived cells compared with. subcutaneous fat-derived cells - Distinguishing properties of mesenchymal stem cells in humans
Higher chondrogenic potential of fibrous synovium- and adipose synovium-derived cells compared with. subcutaneous fat-derived cells - Distinguishing properties of mesenchymal stem cells in humans
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DOI：
10.1002/art.21651
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发表时间：
2006-03-01
影响因子：
--
通讯作者：
Sekiya, I
中科院分区：
文献类型：
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作者：
Mochizuki, T;Muneta, T;Sekiya, I
Objective.来自滑膜的间充质干细胞比来自骨髓、骨膜、脂肪和肌肉的间充质干细胞具有更大的增殖和软骨形成潜力。本研究旨在比较纤维性滑膜和脂肪性滑膜（滑膜和滑膜下的成分），以确定哪种更适合间充质干细胞的来源，尤其是软骨再生，并检查脂肪性滑膜衍生细胞、纤维性滑膜衍生细胞和皮下脂肪衍生细胞的特征，以确定它们的相似性。从4名年轻供体和4名老年供体中收获人纤维滑膜、脂肪滑膜和皮下脂肪。消化后，将有核细胞以被认为适当的密度铺板，以最大速率扩增，而不发生集落间接触。比较细胞的表面抗原表位、增殖能力、克隆形成率以及成软骨、成骨和成脂分化潜能。与皮下脂肪来源的细胞相比，纤维滑膜和脂肪滑膜来源的细胞在STRO-1和CD 106中较高，在CD 10中较低。与皮下脂肪来源的细胞相比，来自纤维和脂肪滑膜的细胞在混合群体和单细胞来源的培养物中具有更高的增殖潜力和集落形成效率。在软骨形成试验中，纤维滑膜和脂肪滑膜来源的细胞颗粒比皮下脂肪细胞颗粒产生更多的软骨基质。纤维性滑膜和脂肪性滑膜来源的细胞的成骨能力也更高，而成脂潜力在3个群体中几乎难以区分。年轻和老年供体的细胞分化潜能相似。来自纤维滑膜和脂肪滑膜的细胞表现出相当的软骨形成潜力。与皮下脂肪来源的细胞相比，脂肪滑膜来源的细胞更类似于纤维滑膜来源的细胞。
Objective. Mesenchymal stem cells from synovium have a greater proliferation and chondrogenic potential than do those from bone marrow, periosteum, fat, and muscle. This study was undertaken to compare fibrous synovium and adipose synovium (components of the synovium with subsynovium) to determine which is a more suitable source for mesenchymal stem cells, especially for cartilage regeneration, and to examine the features of adipose synovium-derived cells, fibrous synovium-derived cells, and subcutaneous fat-derived cells to determine their similarities.Methods. Human fibrous synovium, adipose synovium, and subcutaneous fat were harvested from 4 young donors and 4 elderly donors. After digestion, the nucleated cells were plated at a density considered proper to expand at a maximum rate without colony-to-colony contact. The surface epitopes, proliferative capacity, cloning efficiency, and chondrogenic, osteogenic, and adipogenic differentiation potentials of the cells were compared.Results. Fibrous synovium- and adipose synovium-derived cells were higher in STRO-1 and CD106 and lower in CD10 compared with subcutaneous fat-derived cells. Cells derived from fibrous and adipose synovium had higher proliferative potential and colony-forming efficiency compared with subcutaneous fat-derived cells, both in mixed-population and in single-cell-derived cultures. In chondrogenic assays, pellets from fibrous synovium- and adipose synovium-derived cells produced more cartilage matrix than did cell pellets from subcutaneous fat. Osteogenic ability was also higher in fibrous synovium- and adipose synovium-derived cells, whereas adipogenic potential was nearly indistinguishable among the 3 populations. Differentiation potential of the cells was similar between young and elderly donors.Conclusion. Cells derived from the fibrous synovium and from the adipose synovium demonstrate comparable chondrogenic potential. Adipose synovium-derived cells are more similar to fibrous synovium-derived cells than to subcutaneous fat-derived cells.