Human monocyte ATP-induced IL-1 beta posttranslational processing is a dynamic process dependent on in vitro growth conditions
Human monocyte ATP-induced IL-1 beta posttranslational processing is a dynamic process dependent on in vitro growth conditions
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DOI：
10.1002/jlb.62.2.227
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发表时间：
1997-08-01
影响因子：
5.5
通讯作者：
Gabel, CA
中科院分区：
文献类型：
--
作者：
Laliberte, RE;Perregaux, DG;Gabel, CA
尽管有很大的生产能力，新鲜分离的脂多糖（LPS）激活的人单核细胞仅释放一小部分新合成的白细胞介素（IL）-1 β进入培养基。细胞外ATP通过表面P-2 Z型嘌呤受体起作用，增加细胞因子翻译后加工。为了探索这种ATP反应是否受到培养条件的影响，在不存在和存在各种培养基成分（包括胎牛和人血清以及重组人细胞因子）的情况下，将单核细胞维持不同的时间段。单核细胞产生放射性标记的pro-IL-1 β的能力，以响应LPS和ATP刺激后的posterior过程的原细胞因子的影响，通过培养时间和特定的培养基成分的存在。这些观察结果表明，ATP促进人单核细胞IL-1 β翻译后加工的能力是一个受细胞因子和/或生长因子调节的动态过程。单核细胞/巨噬细胞ATP反应性的变化可能为体内IL-1生物活性的控制提供重要的调节机制。
Despite a large production capacity, freshly isolated lipopolysaccharide (LPS)-activated human monocytes release only a small percentage of their newly synthesized interleukin (IL)-1 beta into the medium. Extracellular ATP, acting via surface P-2Z-type purino-receptors, increases cytokine posttranslational processing. To explore whether this ATP response was affected by culture conditions, monocytes were maintained for different time periods in the absence and presence of various media components including fetal bovine and human sera and recombinant human cytokines. The ability of monocytes to produce radiolabeled pro-IL-1 beta in response to LPS and to posttranslationally process the procytokine after ATP stimulation was affected both by time in culture and by the presence of specific media components. These observations indicate that ATP's ability to promote human monocyte IL-1 beta posttranslational processing is a dynamic process that is subject to regulation by cytokines and/or growth factors. Changes in monocyte/macrophage ATP responsiveness may provide an important regulatory mechanism for the control of IL-1 biological activity in vivo.