The extent of the uptake of plasmid into the skin determines the immune responses induced by a DNA vaccine applied topically onto the skin.
The extent of the uptake of plasmid into the skin determines the immune responses induced by a DNA vaccine applied topically onto the skin.
复制标题
皮肤吸收质粒的程度决定了局部应用于皮肤的 DNA 疫苗诱导的免疫反应。
DOI：
10.1111/j.2042-7158.2010.01219.x
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Cui,Zhengrong
中科院分区：
文献类型：
--
作者：
Yu,Zhen;Chung,Woon-Gye;Sloat,BrianR;Lohr,ChristianeV;Weiss,Richard;Rodriguez,BLeticia;Li,Xinran;Cui,Zhengrong
ObjectivesNon-invasive immunization by application of plasmid DNA topically onto the skin is an attractive immunization approach. However, the immune responses induced are generally weak. Previously, we showed that the antibody responses induced by topical DNA vaccine are significantly enhanced when hair follicles in the application area are induced into the anagen (growth) stage by hair plucking. In the present study, we further investigated the mechanism of immune enhancement.MethodsThree different methods – hair plucking or treatment with retinoic acid (RA) or O-tetradecanoylphorbol-13-acetate (TPA) – were used to induce mice hair follicles into the anagen stage before they were dosed with aβ-galactosidase-encoding plasmid, and the specific antibody responses induced were evaluated.Key findingsThe hair-plucking method was more effective at enhancing the resultant antibody responses. Treatment with RA or TPA caused more damage to the skin and induced more severe local inflammation than hair plucking. However, hair plucking was most effective at enhancing the uptake or retention of the DNA in the application area.ConclusionsThe uptake of plasmid DNA in the application area correlated with the antibody responses induced by a topically applied DNA.
