Distinct regulation of MHC molecule expression on astrocytes and microglia during viral encephalomyelitis
Distinct regulation of MHC molecule expression on astrocytes and microglia during viral encephalomyelitis
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DOI：
10.1002/glia.20538
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发表时间：
2007-08-15
期刊：
影响因子：
6.2
通讯作者：
Bergmann, Cornelia C.
中科院分区：
文献类型：
--
作者：
Hamo, Ludwig;Stohlman, Stephen A.;Bergmann, Cornelia C.
通过比较星形胶质细胞和小胶质细胞上主要组织相容性复合物分子的上调和保留，评估了病毒诱导的炎症期间神经胶质细胞与 T 细胞的潜在相互作用。在星形胶质细胞特异性神经胶质原纤维酸性蛋白启动子的控制下表达绿色荧光蛋白的转基因小鼠被嗜神经冠状病毒感染，以利用流式细胞术促进星形胶质细胞和小胶质细胞的表型表征。成人中枢神经系统中的星形胶质细胞上调 I 类表面表达，尽管与小胶质细胞相比延迟。与小胶质细胞上的有效上调相比，星形胶质细胞上几乎检测不到 II 类。两种神经胶质细胞类型中的最大 MHC 表达与 IFN-γ 水平和淋巴细胞积累相关。尽管病毒清除后 IFN-γ 下降，但神经胶质细胞上的 MHC 分子表达仍持续存在。这些数据证明了病毒诱导的炎症后体内小胶质细胞和星形胶质细胞对 I 类和 II 类表达的不同调节。此外，病毒清除后 MHC 表达延长意味着有可能持续呈递。 (C) 2007 Wiley-Liss, Inc.
The potential interplay of glial cells with T cells during viral induced inflammation was assessed by comparing major histocompatibility complex molecule upregulation and retention on astrocytes and microglia. Transgenic mice expressing green fluorescent protein under control of the astrocytespecific glial fibrillary acidic protein promoter were infected with a neurotropic coronavirus to facilitate phenotypic characterization of astrocytes and microglia using flow cytometry. Astrocytes in the adult central nervous system up-regulated class I surface expression, albeit delayed compared with microglia. Class II was barely detectable on astrocytes, in contrast to potent up-regulation on microglia. Maximal MHC expression in both glial cell types correlated with IFN-gamma levels and lymphocyte accumulation. Despite a decline of IFN-gamma concomitant to virus clearance, MHC molecule expression on glia was sustained. These data demonstrate distinct regulation of both class I and class II expression by microglia and astrocytes in vivo following viral induced inflammation. Furthermore, prolonged MHC expression subsequent to viral clearance implies a potential for ongoing presentation. (C) 2007 Wiley-Liss, Inc.