Use of soft hydrothermal processing to improve and recycle bedding for laboratory animals
Use of soft hydrothermal processing to improve and recycle bedding for laboratory animals
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DOI：
10.1258/la.2007.007065
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发表时间：
2008-10-01
影响因子：
2.4
通讯作者：
Kasai, N.
中科院分区：
文献类型：
--
作者：
Miyamoto, T.;Li, Z.;Kasai, N.
实验室啮齿动物的笼垫会影响动物的健康，从而影响实验数据。此外，大量含有排泄物的旧被褥作为医疗废物从生命科学研究所和养殖公司排出。我们开发了一个突破性的系统，通过应用高温高压干蒸汽的软水热工艺来改善新鲜垫层和回收使用过的垫层。该系统可以去除有害的有机成分和影响动物新陈代谢的芳香烃。本研究的目的是评估的化学和物理性能的改进新鲜垫料和回收的旧垫料处理的系统。结果表明，在0.35 MPa和140 ℃下处理120 mill的新鲜垫料中去除了68-99%的主要芳烃（“改进的垫料”）。此外，59.4-99.0%的来自排泄物的主要有害有机化合物从在0.45 MPa和150 ℃下处理60分钟的用过的垫料中去除（“再循环垫料”）。软水热处理增加了床层表面酸性官能团的数量，使其对氨气有较高的吸附效率。微生物、重金属和农药等有害物质降至检测限以下。结果清楚地表明，改进和回收的垫料对实验室啮齿动物更安全，并有可能改善用于饲养实验室动物的一级和二级围栏（例如笼子和动物房）的条件。这个过程可能是最先进的技术之一，提供了一种替代软木和其他床上用品，通过回收使用过的床上用品和减少动物设施的床上用品废物来节省费用。
Cage bedding for laboratory rodents can influence animal wellbeing and thus the experimental data. In addition, a large amount of used bedding containing excrement is discharged as medical waste from life science institutes and breeding companies. We developed a ground-breaking system to improve fresh bedding and recycle used bedding by applying a soft hydrothermal process with high-temperature and high-pressure dry steam. The system removes both harmful organic components and aromatic hydrocarbons that can affect animals' metabolism. The purpose of the present study was to evaluate the chemical and physical properties of the improved fresh bedding and the recycled Used bedding treated by the system. The results showed that 68-99% of the predominant aromatic hydrocarbons were removed from fresh bedding treated at 0.35 MPa and 140 degrees C for 120 mill ('improved bedding'). in addition, 59.4-99.0% Of predominant harmful organic compounds derived from excrement were removed from used bedding treated at 0.45 MPa and 150 degrees C for 60 min ('recycled bedding'). The soft hydrothermal treatment increased the number of acidic functional groups on the bedding Surface and gave it the high adsorptive efficiency of ammonia gas. Harmful substances such as microorganisms, heavy metals and pesticides decreased below the detection limit. The results clearly showed that the improved and recycled bedding is safer for laboratory rodents and has the potential to ameliorate conditions in primary and secondary enclosures (e.g. cages and animal rooms) used for maintaining laboratory animals. This process may be one of the most advanced techniques in providing an alternative to softwood and other bedding, economizing through the recycling of used bedding and reducing bedding waste from animal facilities.