Fungal communities of lichen-dominated biological soil crusts: Diversity, relative microbial biomass, and their relationship to disturbance and crust cover
Fungal communities of lichen-dominated biological soil crusts: Diversity, relative microbial biomass, and their relationship to disturbance and crust cover
复制标题
DOI：
10.1016/j.jaridenv.2010.05.033
复制
发表时间：
2010-10-01
影响因子：
2.7
通讯作者：
Garcia-Pichel, Ferran
中科院分区：
文献类型：
--
作者：
Bates, Scott T.;Nash, Thomas H., III;Garcia-Pichel, Ferran
利用分子方法对美国科罗拉多高原两个地点地衣为主的生物土壤结皮(BSCs)中的真菌群落进行了研究，以调查它们的多样性和丰度与细菌的多样性和丰度，以及这些参数与整个土壤结皮覆盖和人为干扰的存在之间的对应关系。利用聚合酶链式反应扩增的核糖体基因的变性梯度凝胶电泳(DGGE)指纹图谱和测序分析真菌群落的多样性和组成。针对真菌和细菌的定量聚合酶链式反应被用来评估相对微生物密度。研究了两个土壤性质相似的地点，这两个地点都含有以地衣为主的发育良好的生物干细胞。结果表明，虽然这些生物干细胞中存在相当多的真菌多样性，远远高于以前确定的以蓝藻为主的结壳，但真菌贡献的生物量较少，与细菌同行相比多样性较小。地衣占优势的生物干细胞中真菌多样性与干扰呈负相关，与结皮盖度呈正相关。(C)2010爱思唯尔有限公司。保留所有权利。
Molecular methodologies were used to characterize fungal communities associated with lichen-dominated biological soil crusts (BSCs) at two sites on the Colorado Plateau (USA) in order to investigate their diversity and abundance, in relation to that of bacteria, as well as how these parameters corresponded to overall soil crust cover and the presence of anthropogenic disturbance. Fungal community diversity and composition were assessed with denaturing gradient gel electrophoresis (DGGE) fingerprinting of PCR amplified ribosomal genes and by sequencing. Quantitative PCR, specific for fungi as well as bacteria, was used to evaluate relative microbial densities. Two sites with similar soil characteristics, both of which contained well developed BSCs dominated by lichens, were studied. Results indicated that while a considerable diversity of fungi is present within these BSCs, much higher than what has previously been determined for cyanobacteria-dominated crusts, fungi contribute less biomass and are less diverse than their bacterial counterparts. Fungal diversity in lichen-dominated BSCs was negatively correlated with disturbance and positively correlated with crust cover. (C) 2010 Elsevier Ltd. All rights reserved.