Rps8 maps to a resistance gene rich region on soybean molecular linkage group F
Rps8 maps to a resistance gene rich region on soybean molecular linkage group F
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DOI：
10.2135/cropsci2004.04-0024
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发表时间：
2006-01-01
期刊：
影响因子：
2.3
通讯作者：
Dorrance, AE
中科院分区：
文献类型：
--
作者：
Gordon, SG;Martin, SKS;Dorrance, AE
大豆疫霉根腐病的研究进展。Gerdemann是大豆[Glycine mar（L.）梅尔。]国际吧最近，一个新的基因座，对大豆疫霉菌的抗性，Rps 8，被确定和定位在两个小的大豆群体。本研究的目的是验证Rps 8在更大群体中的基因组定位。利用SSR和RFLP标记对来自“威廉姆斯”（rps 8/rps 8）× PI 399073（Rps 8/Rps 8）杂交的138个F-2：3家系进行了基因型分析。从这组家庭，138和69的表型与P大豆小种I和25，分别。各生理小种的分离比例符合3：1的抗感比例，表明Rps 8为单基因分离。根据SSR和RFLP标记的连锁分析，Rps 8位于该群体的分子连锁群F上。大豆基因组的这一区域包含许多其他抗性基因座以及病原体和害虫抗性QTL。
Phytophthora root and stem rot caused by Phytophthora sojae MA Kaufmann & J.W. Gerdemann is a serious disease of soybean [Glycine mar (L.) Merr.] worldwide. Recently, a new locus for resistance to P sojae, Rps8, was identified and mapped in two small soybean populations. The objective of this study was to verify the genomic location of Rps8 in a larger population. One hundred thirty-eight F-2:3 families from a cross between 'Williams' (rps8/rps8) x PI 399073 (Rps8/Rps8) were genotyped by simple sequence repeat (SSR) and restriction fragment length polymorphism (RFLP) markers. From this set of families, 138 and 69 were phenotyped with P sojae races I and 25, respectively. The segregation ratio for each race fit a 3:1 resistant:susceptible, confirming that Rps8 segregated as a single dominant gene. On the basis of linkage analysis with SSR and RFLP markers, Rps8 was located on molecular linkage group F in this population. This region of the soybean genome contains numerous other resistance gene loci as well as pathogen and pest resistance QTL.