Characterization of a Novel Plantain Asr Gene, MpAsr, that is Regulated in Response to Infection of Fusarium oxysporum f. sp cubense and Abiotic Stresses
Characterization of a Novel Plantain Asr Gene, MpAsr, that is Regulated in Response to Infection of Fusarium oxysporum f. sp cubense and Abiotic Stresses
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DOI：
10.1111/j.1744-7909.2010.00912.x
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发表时间：
2010-03-01
影响因子：
11.4
通讯作者：
Wang, Jin-Fa
中科院分区：
文献类型：
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作者：
Liu, Hai-Yan;Dai, Jin-Ran;Wang, Jin-Fa
Asr（脱落酸、胁迫、成熟诱导）基因通常被广泛的因素上调，包括干旱、寒冷、盐、脱落酸（阿坝）和损伤;除了植物对发育和环境信号的响应之外。我们从低温诱导的大蕉（Musa paradisiaca）叶片的抑制性消减杂交（SSH）cDNA文库中分离到一个Asr基因MpAsr。MpAsr在尖孢镰刀菌F.结果表明，MpAsr在香蕉叶片、果皮和根中均能侵染，表明MpAsr在香蕉对病原菌的反应中起重要作用。此外，一个581 bp的推定启动子区的MpAsr分离通过基因组步行和顺式元件参与非生物胁迫和病原体相关的反应中检测到在同一地区。此外，MpAsr启动子在烟草中表现出阳性活性，并在F. oxysporum F.香蕉黑胫病菌侵染和阿坝、低温、脱水和高盐处理。有趣的是，过表达MpAsr的转基因拟南芥表现出更高的耐旱性，但对F. oxysporum F. [医]古巴细叶藓这些结果表明，MpAsr可能参与植物对非生物胁迫和病原菌攻击的反应。
Asr (abscisic acid, stress, ripening induced) genes are typically upregulated by a wide range of factors, including drought, cold, salt, abscisic acid (ABA) and injury; in addition to plant responses to developmental and environmental signals. We isolated an Asr gene, MpAsr, from a suppression subtractive hybridization (SSH) cDNA library of cold induced plantain (Musa paradisiaca) leaves. MpAsr expression was upregulated in Fusarium oxysporum f. sp. cubense infected plantain leaves, peels and roots, suggesting that MpAsr plays a role in plantain pathogen response. In addition, a 581-bp putative promoter region of MpAsr was isolated via genome walking and cis-elements involved in abiotic stress and pathogen-related responses were detected in this same region. Furthermore, the MpAsr promoter demonstrated positive activity and inducibility in tobacco under F. oxysporum f. sp. cubense infection and ABA, cold, dehydration and high salt concentration treatments. Interestingly, transgenic Arabidopsis plants overexpressing MpAsr exhibited higher drought tolerance, but showed no significant decreased sensitivity to F. oxysporum f. sp. cubense. These results suggest that MpAsr might be involved in plant responses to both abiotic stress and pathogen attack.