A spatiotemporally controllable chemical gradient generator via acoustically oscillating sharp-edge structures.
A spatiotemporally controllable chemical gradient generator via acoustically oscillating sharp-edge structures.
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DOI：
10.1039/c5lc00868a
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发表时间：
2015-11-07
期刊：
影响因子：
6.1
通讯作者：
Huang TJ
中科院分区：
文献类型：
--
作者：
Huang PH;Chan CY;Li P;Nama N;Xie Y;Wei CH;Chen Y;Ahmed D;Huang TJ
产生稳定的、时空可控的浓度梯度的能力对于解决细胞对化学微环境的反应动力学至关重要。在这里，我们展示了一个基于声振荡锐边结构的声流体梯度发生器，它促进了流体的逐步快速混合，以产生可调的动态化学梯度。通过控制压电换能器的驱动电压，我们证明了化学梯度曲线可以方便地改变（空间可控）。此外，通过调整压电换能器的驱动时间，我们产生了脉动的化学梯度（时间可控）。结合这两个特性，我们开发了一个时空可控的梯度发生器。我们的声流梯度发生器的适用性和生物相容性通过演示人类皮肤微血管内皮细胞（HMVEC-d）响应生成的血管内皮生长因子（VEGF）梯度的迁移，以及在长期暴露于声场后保持HMVEC-d细胞的活力来验证。该装置具有制作操作简单、结构紧凑、生物相容性好、可产生时空可调梯度等优点。利用声振荡锐边结构引起的声流效应，演示了一种主动的、时空可控的化学梯度发生器。
The ability to generate stable, spatiotemporally controllable concentration gradients is critical for resolving the dynamics of cellular response to a chemical microenvironment. Here we demonstrate an acoustofluidic gradient generator based on acoustically oscillating sharp-edge structures, which facilitates in a step-wise fashion the rapid mixing of fluids to generate tunable, dynamic chemical gradients. By controlling the driving voltage of a piezoelectric transducer, we demonstrated that the chemical gradient profiles can be conveniently altered (spatially controllable). By adjusting the actuation time of the piezoelectric transducer, moreover, we generated pulsatile chemical gradients (temporally controllable). With these two characteristics combined, we have developed a spatiotemporally controllable gradient generator. The applicability and biocompatibility of our acoustofluidic gradient generator are validated by demonstrating the migration of human dermal microvascular endothelial cells (HMVEC-d) in response to a generated vascular endothelial growth factor (VEGF) gradient, and by preserving the viability of HMVEC-d cells after long-term exposure to an acoustic field. Our device features advantages such as simple fabrication and operation, compact and biocompatible device, and generation of spatiotemporally tunable gradients. An active, spatiotemporally controllable chemical gradient generator is demonstrated utilizing the acoustic streaming effects induced by acoustically oscillating sharp-edge structures.