Intravascular Leukocyte Labeling Refines the Distribution of Myeloid Cells in the Lung in Models of Allergen-induced Airway Inflammation.
Intravascular Leukocyte Labeling Refines the Distribution of Myeloid Cells in the Lung in Models of Allergen-induced Airway Inflammation.
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DOI：
10.4049/immunohorizons.2300059
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发表时间：
2023-12-01
期刊：
影响因子：
--
通讯作者：
Hallstrand TS
中科院分区：
文献类型：
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作者：
Chow YH;Murphy RC;An D;Lai Y;Altemeier WA;Manicone AM;Hallstrand TS
先天免疫细胞群在哮喘中至关重要，其根据组织位置具有不同的功能特征，这增强了在哮喘小鼠模型中表征先天免疫细胞群的精确数量和位置的重要性。在这项研究中，我们对两种哮喘模型中的小鼠进行了死前血管内 (IV) 标记，以区分 IV 室内的先天免疫细胞群与肺组织或气道腔中的先天免疫细胞群。我们对血液、消化的肺组织悬浮液和支气管肺泡灌洗液进行了光谱流式细胞术分析。我们发现 IV 标记的白细胞不会污染支气管肺泡灌洗液的分析，但代表消化肺组织中细胞的很大比例。排除静脉注射白细胞显着提高了肺组织中骨髓细胞评估的准确性，并为嗜酸性粒细胞和中性粒细胞哮喘模型中持续的运输提供了重要的见解。
Innate immune cell populations are critical in asthma with different functional characteristics based on tissue location, which has amplified the importance of characterizing the precise number and location of innate immune populations in murine models of asthma. In this study, we performed premortem intravascular (IV) labeling of leukocytes in mice in two models of asthma to differentiate innate immune cell populations within the IV compartment versus those residing in the lung tissue or airway lumen. We performed spectral flow cytometry analysis of the blood, suspensions of digested lung tissue, and bronchoalveolar lavage fluid. We discovered that IV labeled leukocytes do not contaminate analysis of bronchoalveolar lavage fluid but represent a significant proportion of cells in digested lung tissue. Exclusion of IV leukocytes significantly improved the accuracy of the assessments of myeloid cells in the lung tissue and provided important insights into ongoing trafficking in both eosinophilic and neutrophilic asthma models.