Animal and vegetal poles of the mouse egg predict the polarity of the embryonic axis, yet are nonessential for development.
Animal and vegetal poles of the mouse egg predict the polarity of the embryonic axis, yet are nonessential for development.
复制标题
小鼠卵的动物极和植物极预测了胚胎轴的极性，但对于发育来说并不是必需的。
作者：
M. Ciemerych;D. Mesnard;M. Zernicka
最近的研究表明，早期（植入前）事件可能是重要的极性在哺乳动物胚胎的发展。我们在这里报告谱系追踪实验与绿色荧光蛋白显示，细胞位于靠近或相反的极体在8细胞阶段的小鼠胚胎保留其相同的相对位置在囊胚。因此，它们位于胚泡对称轴的两端，最近的研究表明，胚泡对称轴与植入后胚胎的前后轴相关（见R。J. Weber，R. A. Pedersen，F. Wianny，M. J. Evans和M. Zernicka-Goetz（1999年）。Development 126，5591-5598）。因此，小鼠的胚胎轴可以与极体在8细胞阶段的位置相关，并暗示着与合子的动物-植物轴相关。然而，我们也表明，从2-细胞阶段的卵裂球，其中动物或植物极已被删除构建的嵌合体胚胎可以发育成正常的囊胚，并成为有生育能力的成年小鼠。这也是真实的嵌合体组成的动物或植物极细胞通过正常分裂到8细胞阶段。我们讨论，虽然极性的着床后胚胎可以追溯到8细胞阶段，反过来，以组织的鸡蛋，它不是绝对固定的这个时候。
Recent studies suggest early (preimplantation) events might be important in the development of polarity in mammalian embryos. We report here lineage tracing experiments with green fluorescent protein showing that cells located either near to or opposite the polar body at the 8-cell stage of the mouse embryo retain their same relative positions in the blastocyst. Thus they come to lie on either end of an axis of symmetry of the blastocyst that has recently been shown to correlate with the anterior-posterior axis of the postimplantation embryo (see R. J. Weber, R. A. Pedersen, F. Wianny, M. J. Evans and M. Zernicka-Goetz (1999). Development 126, 5591-5598). The embryonic axes of the mouse can therefore be related to the position of the polar body at the 8-cell stage, and by implication, to the animal-vegetal axis of the zygote. However, we also show that chimeric embryos constructed from 2-cell stage blastomeres from which the animal or the vegetal poles have been removed can develop into normal blastocysts and become fertile adult mice. This is also true of chimeras composed of animal or vegetal pole cells derived through normal cleavage to the 8-cell stage. We discuss that although polarity of the postimplantation embryo can be traced back to the 8-cell stage and in turn to the organisation of the egg, it is not absolutely fixed by this time.
影响因子：
2.7
作者：
Jean-Paul Vincent;John C. Gerhart
通讯作者：
Jean-Paul Vincent;John C. Gerhart
DOI：
10.1242/dev.126.24.5591
发表时间：
1999
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Weber,RJ;Pedersen,RA;Wianny,F;Evans,MJ;Zernicka-Goetz,M
通讯作者：
Zernicka-Goetz,M