Lamellar Spacing in Cuboid Hydroxyapatite Scaffolds Regulates Bone Formation by Human Bone Marrow Stromal Cells
Lamellar Spacing in Cuboid Hydroxyapatite Scaffolds Regulates Bone Formation by Human Bone Marrow Stromal Cells
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DOI：
10.1089/ten.tea.2010.0573
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发表时间：
2011-06-01
影响因子：
4.1
通讯作者：
Saiz, Eduardo
中科院分区：
文献类型：
--
作者：
Mankani, Mahesh H.;Afghani, Shahrzad;Saiz, Eduardo
背景：骨工程的一个主要目标是创造能够承受载荷的大体积结构（支架和干细胞）。支架必须满足两个相互竞争的要求——它们必须具有足够的多孔性以允许营养物质流动以维持细胞活力，同时又必须具有足够的密度以承受负载。我们研究了支架大孔隙率对人骨髓基质细胞形成的骨形成和支架强度的影响。方法：采用机器人铸造法制备硬质立方羟基磷灰石/磷酸三钙支架。保持陶瓷线厚度不变，相邻线之间的距离分别为50、100、200、500或1000 μ m。体外培养的人骨髓基质细胞与支架结合；移植物被放置在免疫缺陷小鼠的皮下。移植器官在9周、18周、23周、38周或50周后被收获。采用组织学和压缩试验分析骨形成和支架强度。结果：对60例移植进行了评价。皮质骨随移植年龄的增加而增加，以500 μ m移植量最大。移植强度在200亩间最大。结论：支架板层间距调节骨形成程度；500亩地的新骨最多，而200亩地的移植骨最强。
Background: A major goal in bone engineering is the creation of large volume constructs (scaffolds and stem cells) that bear load. The scaffolds must satisfy two competing requirements-they need be sufficiently porous to allow nutrient flow to maintain cell viability, yet sufficiently dense to bear load. We studied the effect of scaffold macroporosity on bone formation and scaffold strength, for bone formed by human bone marrow stromal cells.Methods: Rigid cubical hydroxyapatite/tricalcium phosphate scaffolds were produced by robo-casting. The ceramic line thickness was held constant, but the distance between adjacent lines was either 50, 100, 200, 500, or 1000 mu m. Cultured human bone marrow stromal cells were combined with the scaffolds in vitro; transplants were placed into the subcutis of immunodeficient mice. Transplants were harvested 9, 18, 23, 38, or 50 weeks later. Bone formation and scaffold strength were analyzed using histology and compression testing.Results: Sixty transplants were evaluated. Cortical bone increased with transplant age, and was greatest among 500 mu m transplants. In contrast, maximum transplant strength was greatest among 200 mu m transplants.Conclusions: Lamellar spacing within scaffolds regulates the extent of bone formation; 500 mu m yields the most new bone, whereas 200 mu m yields the strongest transplants.