LncRNA CASC2 inhibited the viability and induced the apoptosis of hepatocellular carcinoma cells through regulating miR-24-3p
LncRNA CASC2 inhibited the viability and induced the apoptosis of hepatocellular carcinoma cells through regulating miR-24-3p
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LncRNA CASC2通过调控miR-24-3p抑制肝癌细胞活力并诱导其凋亡
DOI：
10.1002/jcb.26479
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发表时间：
2018-08-01
影响因子：
4
通讯作者：
Xin, Lin
中科院分区：
文献类型：
--
作者：
Fan, Ji-Chang;Zeng, Fei;Xin, Lin
癌症易感性候选基因2（CASC2）是一种近期发现的长链非编码RNA（lncRNA），已被证实在多种人类癌症中发挥众多作用。然而，CASC2在肝细胞癌（HCC）中的参与情况及具体机制仍需进一步阐明。通过实时定量聚合酶链反应（qRT - PCR）测定CASC2和miR - 24 - 3p在肝癌组织及细胞系中的相对表达量。通过细胞活力和细胞凋亡进一步评估CASC2和miR - 24 - 3p对肝癌细胞的影响。进行体内致瘤实验以验证CASC2对肿瘤生长的抑制作用，并进一步阐明miR - 24 - 3p在该机制中的重要作用。与配对的正常组织相比，CASC2在肝癌组织中的相对表达量显著降低，而通过qRT - PCR测定的miR - 24 - 3p在肝癌组织中明显升高。在肝癌细胞系中也发现了这一现象。同时，在肝癌组织中CASC2的表达与miR - 24 - 3p的表达呈负相关（r = - 0.804，P（此处似乎不完整）
Cancer susceptibility candidate 2 (CASC2), a recently discovered long non-coding RNA (lncRNA), was confirmed to play numerous roles in several human cancers. However, the involvement and concrete mechanism of CASC2 in hepatocellular carcinoma (HCC) still need to be further elucidated. The relative expressions of CASC2 and miR-24-3p in HCC tissue and cell lines were determined by quantitative real-time PCR (qRT-PCR). The effects of CASC2 and miR-24-3p on HCC cells were further assessed via cell viability and apoptosis. In vivo tumorigenesis assay was performed to verify the inhibition effect of CASC2 on the tumor growth and further clarify the important role of miR-24-3p in this mechanism. Compared with the paired normal tissues, the relative expression of CASC2 significantly reduced in the HCC tissues, while miR-24-3p as determined by qRT-PCR obviously increased in the HCC tissues. This observation was also found in HCC cell lines. Meanwhile, the expression of CASC2 was negatively related to miR-24-3p expression in the HCC tissues (r=-0.804, P