RENATURATION KINETICS AND THERMAL-STABILITY OF DNA IN AQUEOUS-SOLUTIONS OF FORMAMIDE AND UREA
RENATURATION KINETICS AND THERMAL-STABILITY OF DNA IN AQUEOUS-SOLUTIONS OF FORMAMIDE AND UREA
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DOI：
10.1093/nar/4.10.3537
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发表时间：
1977-01-01
影响因子：
14.9
通讯作者：
HUTTON, JR
中科院分区：
文献类型：
--
作者：
HUTTON, JR
本文报道了甲酰胺和尿素对 DNA 热稳定性和复性动力学影响的系统研究结果。在 0 至 8 摩尔范围内增加尿素浓度，每摩尔的 Tm 降低 2.25°C，每摩尔的复性率降低约 8%。对于氯化钠浓度范围为 0.035M 至 0.88M 的情况，甲酰胺浓度在 0% 至 50% 范围内增加，每百分比甲酰胺的 Tmby 降低 0.60°C。发现盐浓度较高时，Tmon 甲酰胺百分比的依赖性稍大。增加甲酰胺浓度会使复性速率线性降低，每百分比甲酰胺降低 1.1%，因此 50% 甲酰胺中的最佳速率是不含甲酰胺的相同溶液中最佳速率的 0.45。通过考虑复性温度下溶液的粘度来解释尿素和甲酰胺对 DNA 复性速率的影响。
This paper reports the resultos of a systematic study of the effects of formamide and urea on the thermal stability and renaturation kinetics of DNA. Increasing concentrations of urea in the range 0 to 8 molar lowers the Tm by 2.25°C per molar, and decreases the renaturation rate by approximately 8 percent per molar. Increasing concentrations of formamide in the range from 0 to 50 percent lowers the Tmby 0.60°C per percent formamide for sodium chloride concentrations ranging from 0.035M to 0.88M. At higher salt concentrations the dependence of Tmon percent formamide was found to be slightly greater. Increasing formamide concentration decreases the renaturation rate linearly by 1.1% per percent formamide such that the optimal rate in 50% formamide is 0.45 the optimal rate in an identical solution with no formamide. The effects of urea and formamide on the renaturation rates of DNA are explained by consideration of the viscosities of the solutions at the renaturation temperatures.