Effective production of amanitins by two-step cultivation of the basidiomycete, Galerina fasciculata GF-060
Effective production of amanitins by two-step cultivation of the basidiomycete, Galerina fasciculata GF-060
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DOI：
10.1016/s1389-1723(00)88053-6
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发表时间：
2000-01-01
影响因子：
2.8
通讯作者：
Shinozawa, T
中科院分区：
文献类型：
--
作者：
Muraoka, S;Shinozawa, T
尝试使用担子菌 Galerina fasciculata GF-060 发酵来实际生产鹅膏菌素。在液体发酵中，细胞内的α-和γ-鹅膏菌素是主要产物，而α-和β-鹅膏菌素在固体培养的菌丝体中积累。液体发酵中鹅膏菌素的生产受到剩余碳源（特别是葡萄糖和蔗糖）量的强烈影响。在分批培养中，α-鹅膏蕈碱的生产率为1.58mg/l。为了提高生产率，尝试使用无葡萄糖培养基进行替代培养。结果，α-鹅膏蕈碱的最大产量达到了 5.02 毫克/升。这些条件（发酵方式和葡萄糖饥饿）对于生产所有已知类型的鹅膏蕈素是有效的。
Practical production of amanitins was attempted by fermentation using a basidiomycete, Galerina fasciculata GF-060. In liquid fermentation, intracellular alpha- and gamma-amanitins were the main products, while alpha- and beta- amanitins accumulated in solid cultured mycelia. The production of amanitins in liquid fermentation was strongly affected by the amount of the remaining carbon sources (particularly glucose and sucrose). In batch cultivation, the productivity of alpha-amanitin was 1.58 mg/l. To improve the productivity, replacement cultivation using glucose-free medium was attempted. As a result, the maximum production of alpha-amanitin reached 5,02 mg/l. These conditions (fermentation style and glucose starvation) are effective for the production of all the known types of amanitins.