Comparative study between transcriptionally- and translationally-acting adenine riboswitches reveals key differences in riboswitch regulatory mechanisms.
Comparative study between transcriptionally- and translationally-acting adenine riboswitches reveals key differences in riboswitch regulatory mechanisms.
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DOI：
10.1371/journal.pgen.1001278
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发表时间：
2011-01-20
期刊：
影响因子：
4.5
通讯作者：
Lafontaine DA
中科院分区：
文献类型：
--
作者：
Lemay JF;Desnoyers G;Blouin S;Heppell B;Bastet L;St-Pierre P;Massé E;Lafontaine DA
许多细菌的mRNA在转录或翻译水平上受到称为核糖开关的配体结合元件的调控。虽然它们都结合腺嘌呤，但枯草芽孢杆菌和创伤弧菌的腺嘌呤核糖开关分别控制转录和翻译。在这里，我们表明，除了在转录和翻译调制的明显差异，腺嘌呤核糖开关表现出不同的配体结合特性，并出现在不同的监管制度（动力学与热力学）下运行。而B.枯草杆菌pbuE核糖开关完全依赖于腺嘌呤的共转录结合来发挥功能，创伤弧菌添加核糖开关在转录完成后可与腺嘌呤结合并仍进行翻译调控。进一步的研究表明，转录速率对B至关重要。枯草杆菌pbuE核糖开关有效地执行，这与共转录调控一致。我们的研究结果表明，基因调控控制的性质，即转录或翻译，可能有很高的重要性，核糖开关调节机制。细菌的遗传调控主要在转录和翻译水平上进行。最近发现的核糖开关是位于信使RNA的非翻译区的RNA分子，其调节参与小代谢物的运输和代谢的基因的表达。一些核糖开关最近已被证明采用不同的调节机制，但还没有从这些研究中推导出一般的规则。在这里，我们分析了两个腺嘌呤传感核糖开关的枯草芽孢杆菌和创伤弧菌，不同的水平，他们控制基因表达，这是转录和翻译，分别。我们发现，除了在转录和翻译调控方面的明显差异外，两种腺嘌呤核糖开关的核糖开关调控机制也有根本的不同。例如，当腺嘌呤核糖开关从B。枯草芽孢杆菌进行共转录结合以进行基因调控，而创伤弧菌的核糖开关依赖于可逆的配体结合以在mRNA翻译期间实现基因调控。与B的共转录结合一致。枯草核糖开关，我们发现转录暂停是至关重要的基因调控。我们的研究结果表明，基因调控控制的性质，即转录或翻译，可能有很高的重要性，核糖开关调节机制。
Many bacterial mRNAs are regulated at the transcriptional or translational level by ligand-binding elements called riboswitches. Although they both bind adenine, the adenine riboswitches of Bacillus subtilis and Vibrio vulnificus differ by controlling transcription and translation, respectively. Here, we demonstrate that, beyond the obvious difference in transcriptional and translational modulation, both adenine riboswitches exhibit different ligand binding properties and appear to operate under different regulation regimes (kinetic versus thermodynamic). While the B. subtilis pbuE riboswitch fully depends on co-transcriptional binding of adenine to function, the V. vulnificus add riboswitch can bind to adenine after transcription is completed and still perform translation regulation. Further investigation demonstrates that the rate of transcription is critical for the B. subtilis pbuE riboswitch to perform efficiently, which is in agreement with a co-transcriptional regulation. Our results suggest that the nature of gene regulation control, that is transcription or translation, may have a high importance in riboswitch regulatory mechanisms. Bacterial genetic regulation is mostly performed at the levels of transcription and translation. Recently discovered riboswitches are RNA molecules located in untranslated regions of messenger RNAs that modulate the expression of genes involved in the transport and metabolism of small metabolites. Several riboswitches have recently been shown to employ various regulation mechanisms, but no general rules have yet been deduced from these studies. Here, we have analyzed two adenine-sensing riboswitches of Bacillus subtilis and Vibrio vulnificus that differ by the level at which they control gene expression, which is transcription and translation, respectively. We find that, beyond the obvious difference in transcriptional and translational modulation, riboswitch regulation mechanisms of both adenine riboswitches are fundamentally different. For instance, while the adenine riboswitch from B. subtilis performs co-transcriptional binding for gene regulation, the riboswitch from V. vulnificus relies on reversible ligand binding to achieve gene regulation during mRNA translation. In agreement with co-transcriptional binding of the B. subtilis riboswitch, we find that transcriptional pausing is crucial for gene regulation. Our results suggest that the nature of gene regulation control, that is transcription or translation, may have a high importance in riboswitch regulatory mechanisms.
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