An RNA ligase essential for RNA editing and survival of the bloodstream form of Trypanosoma brucei
An RNA ligase essential for RNA editing and survival of the bloodstream form of Trypanosoma brucei
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DOI：
10.1126/science.1058655
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发表时间：
2001-03-16
期刊：
影响因子：
56.9
通讯作者：
Stuart, K
中科院分区：
文献类型：
--
作者：
Schnaufer, A;Panigrahi, AK;Stuart, K
锥虫中的RNA编辑通过一系列由大分子复合物催化的酶促步骤发生。TbMP52蛋白被证明是该复合物的一个组成部分，具有RNA连接酶活性，并且是复合物中的两个腺苷酸化蛋白之一。TbMP52的调节性抑制阻断了编辑，这表明它是编辑复合物的功能组分。这种抑制在寄生虫的血液中是致命的，表明编辑在生命周期的哺乳动物阶段是必不可少的。存在于所有动质体寄生虫中的编辑复合物因此可能是化学治疗靶点。
RNA editing in trypanosomes occurs by a series of enzymatic steps that are catalyzed by a macromolecular complex. The TbMP52 protein is shown to be a component of this complex, to have RNA Ligase activity, and to be one of two adenylatable proteins in the complex. Regulated repression of TbMP52 blocks editing, which shows that it is a functional component of the editing complex. This repression is lethal in bloodforms of the parasite, indicating that editing is essential in the mammalian stage of the life cycle. The editing complex, which is present in all kinetoplastid parasites, may thus be a chemotherapeutic target.