Differential contributions to the transcriptome of duplicated genes in response to abiotic stresses in natural and synthetic polyploids
Differential contributions to the transcriptome of duplicated genes in response to abiotic stresses in natural and synthetic polyploids
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DOI：
10.1111/j.1469-8137.2011.03650.x
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发表时间：
2011-01-01
期刊：
影响因子：
9.4
通讯作者：
Adams, Keith L.
中科院分区：
文献类型：
--
作者：
Dong, Shaowei;Adams, Keith L.
多倍体在植物进化过程中一直存在，当它发生时和在进化过程中会导致基因表达的显著变化。本研究以异源四倍体陆地棉（Gossypium hirsutum）为材料，分析了5种非生物胁迫条件下叶片、根和子叶中60个重复基因的表达模式（热、冷、干旱、高盐和水浸），在一个或多个胁迫处理下，超过70%的基因在重复的相对表达水平上表现出胁迫诱导的变化，并且在处理之间经常发生变异。12对在不同的非生物胁迫处理表现出相反的表达水平的变化。胁迫诱导的表达变化发生在合成异源多倍体，但有一点对应的模式之间的天然和合成polyploids.Our研究结果表明，非生物胁迫条件下可以有相当大的影响重复基因的表达在一个多倍体，与基因，应力和器官类型的影响。环境胁迫下的差异表达可能是多倍体中某些重复基因得以保存的一个因素。
P>Polyploidy has occurred throughout plant evolution and can result in considerable changes to gene expression when it takes place and over evolutionary time. Little is known about the effects of abiotic stress conditions on duplicate gene expression patterns in polyploid plants.We examined the expression patterns of 60 duplicated genes in leaves, roots and cotyledons of allotetraploid Gossypium hirsutum in response to five abiotic stress treatments (heat, cold, drought, high salt and water submersion) using single-strand conformation polymorphism assays, and 20 genes in a synthetic allotetraploid.Over 70% of the genes showed stress-induced changes in the relative expression levels of the duplicates under one or more stress treatments with frequent variability among treatments. Twelve pairs showed opposite changes in expression levels in response to different abiotic stress treatments. Stress-induced expression changes occurred in the synthetic allopolyploid, but there was little correspondence in patterns between the natural and synthetic polyploids.Our results indicate that abiotic stress conditions can have considerable effects on duplicate gene expression in a polyploid, with the effects varying by gene, stress and organ type. Differential expression in response to environmental stresses may be a factor in the preservation of some duplicated genes in polyploids.