Growth factors interact with estradiol and gonadotropins in the regulation of ovarian cancer cell growth and growth factor receptors.
Growth factors interact with estradiol and gonadotropins in the regulation of ovarian cancer cell growth and growth factor receptors.
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发表时间：
1993
影响因子：
3.1
通讯作者：
J. Wimalasena;D. Meehan;R. Dostal;Foster Js;Mark R. Cameron;M. Smith
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文献类型：
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作者：
J. Wimalasena;D. Meehan;R. Dostal;Foster Js;Mark R. Cameron;M. Smith
控制上皮性卵巢癌（OV-Ca）细胞生长的因素仍不明确。流行病学证据表明，促性腺激素和雌激素可能是重要的发展OVCa。我们已经建立了永久性培养的OVCa细胞从肠系膜转移的卵巢乳头状腺癌。皮摩尔浓度的17 β-E2（但不是17 α-E2）和0.1至1微克/毫升的hCG（但不是hFSH）使这些细胞的生长增加20-60%（p < 0.01）。EGF和IGF-1都增加了细胞生长，并且观察到细胞数量增加3至5倍的最大效果。在对EGF和IGF-1的生长反应为次最大的条件下，E2（0.1至100 nM）在对IGF-1和EGF的生长反应中产生浓度相关的增加。10 nM E2增加了对EGF和IGF-1的反应超过2倍。在组合中，E2/hCG增强了对EGF的生长反应，但对IGF-1没有。EGF受体的Ka增加约2倍（p < 0.01），IGF-1受体/细胞的数量增加约50%（p < 0.01）。虽然hCG对EGF受体结合参数没有影响，但它增加了IGF-1受体的Ka，并增强了E2对IGF-1受体数量的影响。这些研究表明，E2和hCG可能调节OVCa细胞对IGF-1和EGF的细胞生长反应。这些变化可能至少部分地通过调节两种生长因子受体的特性来实现。
The factors controlling cell growth in epithelial ovarian cancer (OV Ca) remain poorly defined. Epidemiological evidence suggests that gonadotropins and estrogens may be important in the development of OV Ca. We have established permanent cultures of OV Ca cells from a mesenteric metastasis of an ovarian papillary adenocarcinoma. The growth of these cells was increased 20-60% (p < 0.01) by picomolar concentrations of 17 beta-E2 (but not by 17 alpha-E2) and 0.1 to 1 microgram/mL of hCG (but not hFSH). Both EGF and IGF-1 increased cell growth, and a maximal effect of 3- to 5-fold increase in cell number was observed. Under conditions in which the growth responses to EGF and IGF-1 were submaximal, E2 (0.1 to 100 nM) produced a concentration related increase in the growth response to IGF-1 and EGF. Ten nM E2 increased the responses to EGF and IGF-1 more than 2-fold. In combination, E2/hCG enhanced the growth response to EGF but not to IGF-1. Estradiol increased the Ka of the EGF receptor approximately 2-fold (p < 0.01) and increased the number of receptors/cell for IGF-1 approximately 50% (p < 0.01). While hCG had no effect on EGF receptor binding parameters, it increased the Ka of the IGF-1 receptor and enhanced the effect of E2 on IGF-1 receptor number. These studies suggest that E2 and hCG may regulate cell growth responses of OV Ca cells to IGF-1 and EGF. These changes may be exerted at least partly through modulation of properties of receptors for the two growth factors.