Microampere Electric Current Causes Bacterial Membrane Damage and Two-Way Leakage in a Short Period of Time
Microampere Electric Current Causes Bacterial Membrane Damage and Two-Way Leakage in a Short Period of Time
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DOI：
10.1128/aem.01015-20
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发表时间：
2020-08-01
影响因子：
4.4
通讯作者：
Wang, Yong
中科院分区：
文献类型：
--
作者：
Krishnamurthi, Venkata Rao;Rogers, Ariel;Wang, Yong
物理试剂，如低电压和电流，最近由于其杀菌能力而获得了抗微生物处理的关注。虽然微安电流被证明可以抑制细菌的生长，但目前还不清楚微安电流在多大程度上破坏了细菌膜。在这里，我们研究了由微安电流（= 5 mA）引起的膜损伤和双向泄漏>= 72 h导致细菌膜的显著损伤，这可能导致细胞内容物的泄漏和有毒物质通过受损膜的流入。然而，目前尚不清楚膜损伤和双向（即，向内和向外）泄漏是由较低的（即，微安）电流。在这项工作中，我们着手回答这个问题。我们观察到膜损伤是由微安电流在半小时内引起的，这允许离子、小分子和蛋白质的双向泄漏。
Physical agents, such as low electric voltage and current, have recently gained attention for antimicrobial treatment due to their bactericidal capability. Although microampere electric current was shown to suppress the growth of bacteria, it remains unclear to what extent the microampere current damaged the bacterial membrane. Here, we investigated the membrane damage and two-way leakage caused by microampere electric current (= 5 mA) for >= 72 h led to significant damage of the bacterial membrane, which likely resulted in leakage of cellular contents and influx of toxic substances through the damaged membrane. However, it remains unclear to what extent membrane damage and two-way (i.e., inward and outward) leakage are caused by lower (i.e., microampere) electric current in a shorter time frame. In this work, we set out to answer this question. We observed that the membrane damage was caused by microampere electric current in half an hour, which allowed two-way leakage of ions, small molecules, and proteins.