Hydrogen-rich saline solution attenuates renal ischemia-reperfusion injury
Hydrogen-rich saline solution attenuates renal ischemia-reperfusion injury
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DOI：
10.1007/s00540-010-0942-1
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发表时间：
2010-08-01
影响因子：
2.8
通讯作者：
Noguchi, Takayuki
中科院分区：
文献类型：
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作者：
Shingu, Chihiro;Koga, Hironori;Noguchi, Takayuki
肾缺血再灌注（I/R）是急性肾损伤的重要原因，在各种手术中不可避免，包括肾移植、肾动脉血管重建术、肾部分切除术和肾上主动脉瘤的治疗。I/R损伤的加重由活性氧（ROS）介导。最近的一项研究表明，氢具有抗氧化性能。在本研究中，我们在啮齿动物模型中检验了富氢盐溶液（HRSS）减轻肾I/R损伤的假设。治疗24小时后，我们进行了组织学检查和透射电子显微镜，并测量血清中的8-OHdG.Histological分析显示，显着减少间质充血，水肿，炎症和出血肾组织收获HRSS治疗后24小时相比，生理盐水给药。肾I/R损伤，这导致改变线粒体形态，也被HRSS抑制。此外，血清中8-OHdG水平显着降低HRSS治疗的大鼠和肾I/R。这些保护作用可能是由于HRSS的抗氧化性能。这些结果表明，HRSS是一个潜在的治疗候选人治疗各种I/R疾病。
Renal ischemia-reperfusion (I/R), an important cause of acute kidney injury, is unavoidable during various types of operations, including renal transplantation, surgical revascularization of the renal artery, partial nephrectomy, and treatment of suprarenal aortic aneurysms. Exacerbation of I/R injury is mediated by reactive oxygen species (ROS). A recent study has shown that hydrogen has antioxidant properties. In this study, we tested the hypothesis that a hydrogen-rich saline solution (HRSS) attenuates renal I/R injury in a rodent model.Rats were treated with an intravenous injection of HRSS or control saline solution followed by renal I/R. After 24 h of treatment, we performed a histological examination and transmission electron microscopy, and measured serum levels of 8-OHdG.Histological analysis revealed a marked reduction of interstitial congestion, edema, inflammation, and hemorrhage in renal tissue harvested 24 h after HRSS treatment compared to saline administration. Renal I/R injury, which led to altered mitochondrial morphology, was also inhibited by HRSS. Furthermore, serum 8-OHdG levels were significantly lower in rats treated with HRSS and subjected to renal I/R.These protective effects were likely due to the antioxidant properties of HRSS. These results suggest that HRSS is a potential therapeutic candidate for treating various I/R diseases.