COVID-19 surveillance in Southeastern Virginia using wastewater-based epidemiology
COVID-19 surveillance in Southeastern Virginia using wastewater-based epidemiology
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DOI：
10.1016/j.watres.2020.116296
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发表时间：
2020-11-01
期刊：
影响因子：
12.8
通讯作者：
Gonzalez, Dana
中科院分区：
文献类型：
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作者：
Gonzalez, Raul;Curtis, Kyle;Gonzalez, Dana
基于水的流行病学（WBE）已被用于分析污水处理厂（WWTP）进水中的标志物，以表征社区内新出现的化学品、药物使用模式或疾病传播。当与临床数据集结合时，这种方法特别有助于理解新型冠状病毒疾病-19（COVID-19）等疾病的爆发。在这项研究中，三个RT-ddPCR检测（N1，N2，N3）严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的RNA检测每周样本从9个污水处理厂在弗吉尼亚州东南部。在采样的最初几周，SARS-CoV-2的检测是零星的。从3月中旬到7月下旬，样本中RNA的检测频率和总体浓度增加。在为期21周的研究中，在检测到病毒RNA的样本中，SARS-CoV-2的浓度范围为10（1）至10（4）拷贝100 mL（-1）。在研究期间，几个污水处理厂服务区的人口标准化负荷率波动与已知的疫情一致。在这里，我们提出了几种方法，数据可以在空间和时间上呈现，以最大限度地利用公共卫生官员。随着COVID-19大流行的减弱，社区可能会看到小规模局部疫情的发生率增加。在这些情况下，WBE可以用作预筛选工具，以更好地针对资源有限的社区的临床测试需求。(c)2020年，任作家。由Elsevier Ltd.发布。这是CC BY-NC-ND许可下的开放获取文章。(http（地址：//creativecommons.org/licenses/by-nc-nd/4.0/）
Wastewater-based epidemiology (WBE) has been used to analyze markers in wastewater treatment plant (WWTP) influent to characterize emerging chemicals, drug use patterns, or disease spread within com-munities. This approach can be particularly helpful in understanding outbreaks of disease like the novel Coronavirus disease-19 (COVID-19) when combined with clinical datasets. In this study, three RT-ddPCR assays (N1, N2, N3) were used to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in weekly samples from nine WWTPs in southeastern Virginia. In the first several weeks of sampling, SARS-CoV-2 detections were sporadic. Frequency of detections and overall concentrations of RNA within samples increased from mid March into late July. During the twenty-one week study, SARS-CoV-2 concentrations ranged from 10(1) to 10(4) copies 100 mL(-1) in samples where viral RNA was detected. Fluctuations in population normalized loading rates in several of the WWTP service areas agreed with known outbreaks during the study. Here we propose several ways that data can be presented spatially and temporally to be of greatest use to public health officials. As the COVID-19 pandemic wanes, it is likely that communities will see increased incidence of small, localized outbreaks. In these instances, WBE could be used as a pre-screening tool to better target clinical testing needs in communities with limited resources. (c) 2020 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license. (http://creativecommons.org/licenses/by-nc-nd/4.0/)